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Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
49385pcDNA3 Connexin43-GFP-APEX2Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells
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49386pcDNA3 APEX2-NESExpresses APEX2 in the cytosol of mammalian cells
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66170Vimentin - APEX2 in pECFPAPEX2 fused to the C-terminus human vimentin
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66171APEX2 - Tubulin in pEGFPAPEX2 fused to the N-terminus of alpha tubulin
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66172APEX2 - Actin in pEGFPAPEX2 fused to the N-terminus of human beta actin
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72480mito-V5-APEX2Expresses APEX2 in mitochondria
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72558pTRC-APEX2Expression of cytosolic APEX2 in bacteria
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79055ERM-APEX2Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
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79056APEX2-OMMSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
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79057MICU-APEX2A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
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