PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49385||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5366
- Total vector size (bp) 8039
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert nameConnexin43 - EGFP - APEX2
Insert Size (bp)2673
MutationK14D, W41F, E112K, A134P on soybean APX; improved for proteomics and EM applications
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 Connexin43-GFP-APEX2 was a gift from Alice Ting (Addgene plasmid # 49385 ; http://n2t.net/addgene:49385 ; RRID:Addgene_49385)
For your References section:Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960