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APEX2 - Actin in pEGFP
(Plasmid #66172)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66172 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 3954
  • Total vector size (bp) 5907
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APEX2 - human beta Actin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1953
  • Mutation
    APEX2 contains 4 mutations relative to wt soybean APX: K14D, W41F, E112K, A134P
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag epitope tag (DYKDDDDK) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMVf (CGCAAATGGGCGGTAGGCGTG)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    APEX2 - Actin in pEGFP was a gift from Alice Ting (Addgene plasmid # 66172 ; http://n2t.net/addgene:66172 ; RRID:Addgene_66172)
  • For your References section:

    Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960