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ERM-APEX2
(Plasmid #79055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79055 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLX304
  • Backbone size w/o insert (bp) 7740
  • Total vector size (bp) 8694
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APEX2 anchored to the cytosolic face of the ER membrane
  • Species
    Soybean
  • Insert Size (bp)
    954
  • Promoter CMV immearly promotor
  • Tags / Fusion Proteins
    • Residues 1-27 of P450 oxidase 2C1 (N terminal on insert)
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Additional publication for this plasmid:
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung et. al. Elife 2017 https://www.ncbi.nlm.nih.gov/pubmed/28441135

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ERM-APEX2 was a gift from Alice Ting (Addgene plasmid # 79055 ; http://n2t.net/addgene:79055 ; RRID:Addgene_79055)
  • For your References section:

    Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960