PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||79055||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7740
- Total vector size (bp) 8694
Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameAPEX2 anchored to the cytosolic face of the ER membrane
Insert Size (bp)954
- Promoter CMV immearly promotor
/ Fusion Proteins
- Residues 1-27 of P450 oxidase 2C1 (N terminal on insert)
- V5 (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-forward (Common Sequencing Primers)
Additional publication for this plasmid:
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung et. al. Elife 2017 https://www.ncbi.nlm.nih.gov/pubmed/28441135
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ERM-APEX2 was a gift from Alice Ting (Addgene plasmid # 79055 ; http://n2t.net/addgene:79055 ; RRID:Addgene_79055)
For your References section:Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960