PurposeExpression of cytosolic APEX2 in bacteria
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72558||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2282
- Total vector size (bp) 1509
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsFor expression and purification from bacterial cells, it is recommended to supplement cultures with 1 mM 5-aminolevulinic acid after induction with IPTG, with subsequent growth at room temperature to promote higher heme incorporation.
MutationA134P relative to APEX
/ Fusion Protein
- His6 (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer AGCTGTTGACAATTAATCATCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTRC-APEX2 was a gift from Alice Ting (Addgene plasmid # 72558 ; http://n2t.net/addgene:72558 ; RRID:Addgene_72558)
For your References section:Directed evolution of APEX2 for electron microscopy and proximity labeling. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. 10.1038/nmeth.3179 PubMed 25419960