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High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.

Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD
Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
47505pVC297 VEGF Site#1human gRNA expression vector targeting VEGF
47506pVC299 VEGF Site#2human gRNA expression vector targeting VEGF
47507pVC228 VEGF Site#3human gRNA expression vector targeting VEGF
47508pFYF1548 EMX1human gRNA expression vector targeting EMX1
47509pDR366 RNF2human gRNA expression vector targeting RNF2
47510pDR348 FANCFhuman gRNA expression vector targeting FANCF
47511pFYF1320 EGFP Site#1human gRNA expression vector targeting EGFP
47512pFYF1327 EGFP Site#2human gRNA expression vector targeting EGFP
47513pFYF1328 EGFP Site#3human gRNA expression vector targeting EGFP

Antibodies from Article