pVC299 VEGF Site#2
(Plasmid #47506)

Purpose

human gRNA expression vector targeting VEGF
Depositing Lab

Keith Joung
Publication

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623. ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	47506	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
MLM3636
(Search Vector Database)
Backbone manufacturer
Joung lab (Addgene plasmid #43860)
Backbone size w/o insert (bp) 2278
Vector type
Mammalian Expression, CRISPR

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
XL1 Blue
Copy number
Unknown

Gene/Insert

Gene/Insert name
gRNA-VEGF site 2
Species
H. sapiens (human)
Insert Size (bp)
20
Promoter U6

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site BsmBI (not destroyed)
3′ cloning site BsmBI (not destroyed)
5′ sequencing primer hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 2 References

Depositor Comments

Target site: GACCCCCTCCACCCCGCCTC

gRNA expression vector targeting human VEGF for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9)

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pVC299 VEGF Site#2 was a gift from Keith Joung (Addgene plasmid # 47506 ; http://n2t.net/addgene:47506 ; RRID:Addgene_47506)
For your References section:

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623. 10.1038/nbt.2623 PubMed 23792628

pVC299 VEGF Site#2 (Plasmid #47506)