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(Plasmid #43861)


Item Catalog # Description Quantity Price (USD)
Plasmid 43861 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
  • Alt name
  • Species
    streptococcus pyogenes
  • Promoter CMV
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer OK569 (5’-TAGAAGGCACAGTCGAGG-3’)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mammalian codon-optimized Cas9 expression vector for use with MLM3636 (Addgene plasmid# 43860--human cell gRNA expression vector)

Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839

For more information on Joung Lab CRISPR Plasmids please refer to:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JDS246 was a gift from Keith Joung (Addgene plasmid # 43861 ; ; RRID:Addgene_43861)