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PurposeExpresses high-efficiency TEV protease in bacterial cells
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET29b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTEV protease
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SpeciesTobacco etch virus
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MutationT17S, L56V, N68D, I77V, S135G, I138T, S153N, T180A, S219V
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Tag
/ Fusion Protein
- 6xHis (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutations taken from:
Wei et al., 2012. Protein Expression and Purification
Sanchez and Ting, 2019. Nature Methods
van den Berg et al., 2006. Journal of Biotechnology
Cabrita et al., 2007. Protein Science
Kapust et al., 2002. Biochemical and Biophysical Research Communications
The protocol for the purification of 10xHis-SuperTEV is available on protocols.io: dx.doi.org/10.17504/protocols.io.yxmvm2zxng3p/v1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b-10xHis-Super TEV was a gift from EPFL Protein Production and Structure Core Facility (PTPSP) & Florence Pojer (Addgene plasmid # 193833 ; http://n2t.net/addgene:193833 ; RRID:Addgene_193833) -
For your References section:
Purification of 10xHis-SuperTEV. Lau K, Bouchri B, Pojer F. protocols.io, doi: 10.17504/protocols.io.yxmvm2zxng3p/v1 10.17504/protocols.io.yxmvm2zxng3p/v1
