pET28-MBP-super TEV protease
PurposeExpresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171782||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 7295
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)1926
MutationNo internal TEV cleavage site between the MBP and TEV protease. The TEV protease domain contains the following solubility/stability mutations (numbers refer to the standard TEV protease numbering scheme): C19V L56V C110V C130S S135G and S219D
- Promoter T7
/ Fusion Proteins
- Internal His6 tag
- Arg6 tag (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-MBP-super TEV protease was a gift from Mark Howarth (Addgene plasmid # 171782 ; http://n2t.net/addgene:171782 ; RRID:Addgene_171782)
For your References section:DogCatcher allows loop-friendly protein-protein ligation. Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005. 10.1016/j.chembiol.2021.07.005 PubMed 34324879