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AAVS1-Neo-M2rtTA
(Plasmid #60843)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60843 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 8516
  • Total vector size (bp) 10098
  • Vector type
    targeting donor
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    M2rtTA
  • Insert Size (bp)
    782

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Neo
  • Insert Size (bp)
    800

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    M2-rtTA was obtained from Dr. H. Bujard
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-Neo-M2rtTA was a gift from Rudolf Jaenisch (Addgene plasmid # 60843 ; http://n2t.net/addgene:60843 ; RRID:Addgene_60843)
  • For your References section:

    Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome. DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L, Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch R, Gregory PD, Urnov FD. Genome Res. 2010 Aug;20(8):1133-42. doi: 10.1101/gr.106773.110. Epub 2010 May 27. 10.1101/gr.106773.110 PubMed 20508142