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Addgene

piCRg Entry
(Plasmid #58904)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58904 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Low copy
  • Backbone manufacturer
    home made
  • Backbone size w/o insert (bp) 2570
  • Total vector size (bp) 8302
  • Modifications to backbone
    inroduction of the pBR322 (low copy) origin of replication
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hSpCas9
  • Insert Size (bp)
    4101

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The human codon-optimized S. pyogenes Cas9 was amplified by PCR from plasmid pX260 (Cong et al.,Science. 2013). The chimeric U6-gRNA expression cassette was PCR amplified from pX330 (Cong et al.,Science. 2013).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piCRg Entry was a gift from Danwei Huangfu (Addgene plasmid # 58904 ; http://n2t.net/addgene:58904 ; RRID:Addgene_58904)
  • For your References section:

    An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. 10.1016/j.stem.2014.05.018 PubMed 24931489