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pGRG25
(Plasmid #16665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16665 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha in LB + ampicillin, grow at less than 32 degrees C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mTn7::MCS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII, NotI, PacI, and XhoI (not destroyed)
  • 3′ cloning site AvrII, NotI, PacI, and XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information.

This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGRG25 was a gift from Nancy Craig (Addgene plasmid # 16665 ; http://n2t.net/addgene:16665 ; RRID:Addgene_16665)
  • For your References section:

    Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion event. McKenzie GJ, Craig NL. BMC Microbiol. 2006 . 6():39. 10.1186/1471-2180-6-39 PubMed 16646962