pETM41-EcLonA
(Plasmid #38330)

• Depositing Lab: Florian Freimoser
• Publication: Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.

Backbone

• Vector backbone: pETM41
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lon protease fragment
• Alt name: LonA
• Species: Escherichia coli
• Mutation: Polyphosphate binding fragment of Lon protease. After the start methionine, the protein starts at amino acid 287, the protein has a length of 168 amino acids
• Entrez Gene: lon (a.k.a. b0439, ECK0433, capR, deg, dir, lopA, muc)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • MBP (N terminal on backbone)
  • TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See "Nomura, K., Kato, J., Takiguchi, N., Ohtake, H., Kuroda, A., 2004. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli. J. Biol. Chem. 279, 34406–34410." for more information.