We narrowed to 9,753 results for: try

1. pCMVHA E2F1

   Plasmid

   #24225

   Purpose

   Mammalian expression of human E2F1 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

   Plasmid

   #200016

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK007 pME KalTa4

   Plasmid

   #195965

   Purpose

   Gateway pME Gal4 variant

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   KalTA4 (Gal4)
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. ChromosomesMembranesandCytoskeleton

   Plasmid

   #62450

   Purpose

   MXS_chaining vector with 4 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::H2B-TagBFPx3-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::mCherryx3-linker-betaActin-bGHpA
   CMV::Citrinex3-linker-alphaTubulin-bGHpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. anti-FLAG M2 light chain

   Plasmid

   #175358

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 light chain

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 light chain
   

   Tags

   N-terminal secretion signal peptide (MEAPAQLLFLLL…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 768 pME mApple-CAAX

   Plasmid

   #195961

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 764 pME nls-mApple

   Plasmid

   #195962

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple nuclear
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 765 pME nls mCerulean

    Plasmid

    #195958

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 769 pME mCerulean-CAAX

    Plasmid

    #195957

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean-CAAX (membrane)
    

    Use

    Other

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCMV-sp-HPV-TM(ICAM)

    Plasmid

    #196510

    Purpose

    Express HPV antigen epitope with ICAM transmembrane domain

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    a HPV epitope with CD8a stalk linker and ICAM TM domain
    

    Use

    Lentiviral

    Tags

    3xflag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLeAPS-GFP-blast

    Plasmid

    #182230

    Purpose

    Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    GFP-P2A-Blasticidin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMito-MtrxRFP2

    Plasmid

    #172324

    Purpose

    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b-His-TEV-CLIP-tag

    Plasmid

    #167274

    Purpose

    Overexpression of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSN001 pME minprom_mCerulean

    Plasmid

    #195959

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter mCerulean
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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