We narrowed to 18,779 results for: multi
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Plasmid#188732PurposeExpress miniGFP1 and FusionRed in mammalian cellsDepositorInsertminiGFP1
UseAAVTagsFusionRedExpressionMammalianPromoterCAGAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianPromoterU6Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-iCAM-1
Plasmid#205186PurposeThis vector encodes of a synCAM (iCAM1) and GFPDepositorInsertsynCAM iCAM1 and GFP
UseLentiviralExpressionMammalianAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Plasmid#176630PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell linesDepositorInsertEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCl-MCS_miniCMV_EGFP
Plasmid#134984PurposeThe plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vectorDepositorInsertEGFP
UseLentiviralPromoterminCMVAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG646_lexO-HO_LexA-TF_LEU2MX
Plasmid#154815PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsTagsFLAGExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM-Dest
Plasmid#102563PurposePiggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterN/AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)
Plasmid#140226PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistanceDepositorInsertLuciferase + crIFNg
ExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Intb1
Plasmid#205193PurposeThis vector encodes of a synCAM (Intb1) and GFPDepositorInsertsynCAM Intb1 and GFP
UseLentiviralExpressionMammalianAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateExpressionPlantAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRel-Hyg
Plasmid#227582PurposeRelease plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 repliconDepositorInsertCas9
UseCRISPRAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Ecad
Plasmid#205188PurposeThis vector encodes of a synCAM (Ecad) and GFPDepositorInsertsynCAM Ecad and GFP
UseLentiviralExpressionMammalianAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-iCAM-1-Tether
Plasmid#205187PurposeThis vector encodes of a synCAM (iCAM1 Tether) and GFPDepositorInsertsynCAM iCAM1 tether and GFP
UseLentiviralExpressionMammalianAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseMultisite gatewayAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only