1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. p155 pCMV-EGFP/beta-gal

   Plasmid

   #8387

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox; Beta-gal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FBE-Luc

   Plasmid

   #16526

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   FAST-1 binding element (FOXH1 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

3. p144 pSV-EGFP/beta-gal

   Plasmid

   #8386

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox; Beta-gal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

4. p169 pCMV/betaAc-EGFP/beta-gal

   Plasmid

   #8388

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP beta-gal
   

   Use

   Cre/Lox; Beta-gal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

5. p150 pPr-beta-gal

   Plasmid

   #8402

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin promoter (Pbsn Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

6. GST-FAST-FL

   Plasmid

   #16523

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   FAST-1 (FOXH1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

7. p218 pCMV-CREM-L

   Plasmid

   #8396

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   CREM-L
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre, single LOX 511 site

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pEF1a-BirA-V5-neo

   Plasmid

   #100548

   Purpose

   Expresses C-terminal V5-tagged BirA biotin ligase

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   C-terminal V5-tagged BirA
   

   Use

   Tags

   V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTPH418b

   Plasmid

   #185725

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-FOXH1

    Plasmid

    #153125

    Purpose

    Expression of Flag tagged human FOXH1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXH1 (FOXH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-Fast-1 wild-type

    Plasmid

    #16521

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FAST-1 (FOXH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p153 pPr-cre

    Plasmid

    #8403

    Purpose

    Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin driven Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    probasin promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. CMV-Fast-1 H83R

    Plasmid

    #16522

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FAST-1 H83R (FOXH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H83R mutant

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

17. GST-FAST-FH

    Plasmid

    #16524

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FAST-1 (FH domain) (FOXH1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-150. Forkhead domain.

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-SpRY-HF1-ABE8e

    Plasmid

    #185670

    Purpose

    Expresses SpRY-HF1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY-HF1 D10A

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSetB-KanR-LiLac

    Plasmid

    #184569

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    Tags

    His7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge