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163,387 results

Showing: 241 - 260 of 163387 results

  2. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113194-plasmid-map-sequence-id-216314

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  8. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

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  14. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  17. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV9)

    Viral Prep
    #100838-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-Syn-ChR2(H134R)-GFP (AAV8)

    Viral Prep
    #58880-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.
    Promoter
    Syn
    Tags
    GFP
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 163387 results