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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Publication

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pCMV-GFP

Plasmid

#11153

Purpose

Mammalian expression vector for expression of GFP (CMV promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

CMV promoter-EGFP

Tags

EGFP

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

86 Articles

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pCMV-GFP

Plasmid

#11153

Purpose

Mammalian expression vector for expression of GFP (CMV promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

CMV promoter-EGFP

Tags

EGFP

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

86 Articles

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

230 Articles

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pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

230 Articles

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Publication

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Publication

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Publication

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Publication

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

67 Articles

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Publication

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

cas9

Use

CRISPR; Lambda red recombinase

Expression

Bacterial

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

67 Articles

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Publication

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Publication

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

142 Articles

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

142 Articles

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Publication

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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