228,909 results

1. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCas9-EGFP

   Plasmid

   #63592

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang, Phil Sharp

   Article

   Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCas9-EGFP

   Plasmid

   #63592

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang, Phil Sharp

   Article

   Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD3

   Plasmid

   #45604

   Depositor

   Barry L. Wanner

   Article

   Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

   Insert

   FRT-cat-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   R6K

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD3

   Plasmid

   #45604

   Depositor

   Barry L. Wanner

   Article

   Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

   Insert

   FRT-cat-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   R6K

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-hRXRα

   Plasmid

   #135910

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha (RXRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGJJ045

    Plasmid

    #183621

    Purpose

    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXR001: EF1a-CasRx-2A-EGFP

    Plasmid

    #109049

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    CasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. kiCAP-AAV-MyoAAV4A

    Plasmid

    #224448

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDYNSL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge