Addgene: Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.

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Sheng Yang
Jiang et al

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S
Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
62225	pCas	Constitutive expression of cas9 and inducible expression of lambda RED and sgR
62226	pTargetF	Constitutive expression of sgRNA without donor editing template DNA
120423	pSU-araC-Cas9	Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

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