Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S
Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62225 | pCas | Constitutive expression of cas9 and inducible expression of lambda RED and sgR |
| 62226 | pTargetF | Constitutive expression of sgRNA without donor editing template DNA |
| 120423 | pSU-araC-Cas9 | Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system |