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Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S
Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62225pCasConstitutive expression of cas9 and inducible expression of lambda RED and sgR
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62226pTargetFConstitutive expression of sgRNA without donor editing template DNA
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120423pSU-araC-Cas9Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system
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