PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120423||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
- Promoter araBAD
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD Forward (Common Sequencing Primers)
an arabinose-inducible promoter to express Cas9
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSU-araC-Cas9 was a gift from Sheng Yang (Addgene plasmid # 120423 ; http://n2t.net/addgene:120423 ; RRID:Addgene_120423)
For your References section:Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838