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PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKD46
- Total vector size (bp) 6959
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsGrow 30 degrees with Spectinomycin (50 mg/L)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameexo, beta, gam, sgRNA
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKDsgRNA-ack was a gift from Kristala Prather (Addgene plasmid # 62654 ; http://n2t.net/addgene:62654 ; RRID:Addgene_62654) -
For your References section:
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Reisch CR, Prather KL. Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. 10.1038/srep15096 PubMed 26463009
Map uploaded by the depositor.
