Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pKDsgRNA-p15
(Plasmid #62656)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKD46
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow at 30 C with Spectinomycin 50
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    exo, beta, gam, sgRNA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKDsgRNA-p15 was a gift from Kristala Prather (Addgene plasmid # 62656 ; http://n2t.net/addgene:62656 ; RRID:Addgene_62656)
  • For your References section:

    The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli. Reisch CR, Prather KL. Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. 10.1038/srep15096 PubMed 26463009