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pMA7CR_2.0
(Plasmid #73950)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73950 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    cas9
  • Species
    Bacteria
  • Insert Size (bp)
    4107
  • Promoter pTet

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tttgttgcgcgctttatcgc
  • 3′ sequencing primer gcgacacggaaatgttgaat
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    lambda beta
  • Insert Size (bp)
    786
  • Promoter pAra

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tgccatagcatttttatccat
  • 3′ sequencing primer taaggcggatcgcaatagac
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    dam
  • Insert Size (bp)
    837
  • Promoter pAra

Cloning Information for Gene/Insert 3

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gtcgacccacaggaactgat
  • 3′ sequencing primer ccgccaggcaaattctgt
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    recX
  • Insert Size (bp)
    501
  • Promoter pTet

Cloning Information for Gene/Insert 4

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tatctgttgtttgtcggtgaacg
  • 3′ sequencing primer gcggtctgtatttcccagaa
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA7CR_2.0 was a gift from Alex Nielsen (Addgene plasmid # 73950 ; http://n2t.net/addgene:73950 ; RRID:Addgene_73950)
  • For your References section:

    CRMAGE: CRISPR Optimized MAGE Recombineering. Ronda C, Pedersen LE, Sommer MO, Nielsen AT. Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452. 10.1038/srep19452 PubMed 26797514