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E-cadherin-GFP
(Plasmid #28009)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28009 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Geneticin (G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-cadherin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2648
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human Ecadherin gene in pCDNA3.1 we had from Alpha Yap laboratory (University of Queensland in 1998) We inserted GFP at C- terminal of Human Ecadherin in pcDNA3.1
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note the EGFP protein sequence in this plasmid is missing the first two amino acids of the standard EGFP sequence (Met-Val) due to the cloning strategy. There is also a Y238I mutation present at the C-terminus of EGFP. These differences do not affect the fluorescent protein properties as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E-cadherin-GFP was a gift from Jennifer Stow (Addgene plasmid # 28009 ; http://n2t.net/addgene:28009 ; RRID:Addgene_28009)
  • For your References section:

    A dileucine motif targets E-cadherin to the basolateral cell surface in Madin-Darby canine kidney and LLC-PK1 epithelial cells. Miranda KC, Khromykh T, Christy P, Le TL, Gottardi CJ, Yap AS, Stow JL, Teasdale RD. J Biol Chem. 2001 Jun 22. 276(25):22565-72. 10.1074/jbc.M101907200 PubMed 11312273