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Sheng Yang Lab Plasmids


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Title Authors Publication
High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18.
Yang lab plasmids (unpublished)
Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
Hygromycin-resistance vectors for gene expression in Pichia pastoris. Yang J, Nie L, Chen B, Liu Y, Kong Y, Wang H, Diao L Yeast. 2014 Apr;31(4):115-25.
mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. Yang J, Jiang W, Yang S FEMS Yeast Res. 2009 Jun;9(4):600-9. doi: 10.1111/j.1567-1364.2009.00503.x. Epub 2009 Mar 25.
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
Sheng Yang lab plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51625pREDIAI-SceI (Saccharomyces cerevisiae)Bacterial Expression ; Red recombinase and I-SceI endonucleaseHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E. coli genome modification pKD46 SCEI Q0160 Add to Cart
51626pREDKII-SceI (Saccharomyces cerevisiae)Bacterial Expression ; Red recombinase and I-SceI endonucleaseHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli Genetic Modification pKD46 SCEI Q0160 Add to Cart
51627pREDTAII-SceI (Saccharomyces cerevisiae)Bacterial Expression ; Red recombinase and I-SceI endonucleaseHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli Genetic Modification pKD46 SCEI Q0160 Add to Cart
51628pREDTKII-SceI (Saccharomyces cerevisiae)Bacterial Expression ; Red recombinase and I-SceI endonucleaseHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli Genetic Modification pKD46 SCEI Q0160 Add to Cart
51652pMDICICmRgenetic modificationHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli genetic modification pMD18-T Simple Add to Cart
51653pMDISIspcRgenetic modificationHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli genetic modification pMD18-T Simple Add to Cart
51655pMDIAIapramycin antibiotic resistance genegenetic modificationHigh-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. E coli genetic modification pMD18-T Simple Add to Cart
51725pKSI-1Bacterial Expression Yang lab plasmids (unpublished) cloning backbone pBluescript II KS(-) Add to Cart
62225pCascas9 (Other)Bacterial Expression, CRISPR ; Lambda Red recombinaseMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. Constitutive expression of cas9 and inducible expression of lambda RED and sgR pKD46 Add to Cart
62226pTargetFsgRNA (Synthetic)Bacterial Expression, CRISPRMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. Constitutive expression of sgRNA without donor editing template DNA Trc99a Add to Cart
73226pCAS1ylCas9 (Other), sgRNA (Synthetic)Bacterial Expression, CRISPR ; in Y. lipolyticaMultiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27. Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells pMCSCen1 Add to Cart
73228pNICKclos2.0Cas9 nickase (Other), sgRNA to xylR (Synthetic)E.coli - clostridium shuttle vectorCRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052 pXY1 Add to Cart
73639pNICKclos1.0Cas9 nickase (Other), sGRNA to pyrEE.coli-clostridium shuttle vectorCRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824 pIMP1-ptb Add to Cart
73640pdCASclosdCas9 (Other), sgRNA to spo0AE.coli-clostridium shuttle vectorCRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824 pIMP1-ptb Add to Cart
85073pKSCTMFAOX1promoter - mazF - AOX1 tt- Zeocin R gene (Other)Gene insertion/deletion in Pichia pastorismazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Res. 2009 Jun;9(4):600-9. doi: 10.1111/j.1567-1364.2009.00503.x. Epub 2009 Mar 25. Gene insertion/deletion in Pichia pastoris pBluescript II KS(-) Add to Cart
85074pAHYB-GFPGFPYeast ExpressionHygromycin-resistance vectors for gene expression in Pichia pastoris. Yeast. 2014 Apr;31(4):115-25. Expression of GFP in Pichia pastoris pAHYB Add to Cart
85077pGKBYeast ExpressionHygromycin-resistance vectors for gene expression in Pichia pastoris. Yeast. 2014 Apr;31(4):115-25. expression in Pichia pastoris pGAPZB Add to Cart
85542pJYS3_ΔcrtYfCpf1 (Other), crRNA of crtYf (Synthetic)Bacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coliCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive transcription of FnCpf1 and crRNA of crtYf pXMJ19 Add to Cart
85543pJYS2cm_crtYfcrRNA of crtYf (Synthetic)Bacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coliCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance pXMJ19 and pTRCmob Add to Cart
85544pJYS2_crtYfcrRNA of crtYf (Synthetic)Bacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coliCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive transcription of crRNA of crtYf, Spectinomycin resistance pXMJ19 and pTRCmob Add to Cart
85545pJYS1PtacCpf1 (Other), recT (Other)Bacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coliCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter pXMJ19 Add to Cart
85546pJYS1PeftuCpf1 (Other), recT (Other)Bacterial Expression, CRISPR ; Shuttle vector Corynebacterium glutamicum / E. coliCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter pXMJ19 Add to Cart
87447pGHYBYeast ExpressionHygromycin-resistance vectors for gene expression in Pichia pastoris. Yeast. 2014 Apr;31(4):115-25. Pichia pastoris expression plasmid pGHYB Add to Cart
89077pJYS2.1-crtYfcrRNA of crtYFBacterial Expression, CRISPRCRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum. pXMJ19 and pTRCmob Add to Cart
90482pSY6group II intron and ItrA (Other)Bacterial Expression Sheng Yang lab plasmids (unpublished) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum pIMP1-ptb Add to Cart
90485pIMP1-PthlBacterial Expression Sheng Yang lab plasmids (unpublished) Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052 pIMP1-Pptb Add to Cart
90486pIMP1-PadcBacterial Expression Sheng Yang lab plasmids (unpublished) Plasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052 pIMP1-Pthl Add to Cart
90487pXY1Bacterial Expression Sheng Yang lab plasmids (unpublished) Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052 pIMP1-Pthl Add to Cart
90488pXY1-spcBacterial Expression Sheng Yang lab plasmids (unpublished) Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052 pXY1 Add to Cart
90489pXY1-spc-ptbsceC (Other)Bacterial Expression Sheng Yang lab plasmids (unpublished) pXY1-spc derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium pXY1-spc Add to Cart
90490pSY14-ptbsceC (Other)Bacterial Expression Sheng Yang lab plasmids (unpublished) pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium pSY8 Add to Cart