PurposeMammalian expression of flag-tagged SIRT1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1791||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2877
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2314
GenBank IDNM_012238 NP_036370
Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The HindIII site used for cloning this plasmid is not unique. There is an additional HindIII site at nucleotide 1569 of the NCBI Reference Sequence: NM_012238.4. There is a unique SacI site at the 5' end of the insert that could be used for cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag-SIRT1 was a gift from Michael Greenberg (Addgene plasmid # 1791 ; http://n2t.net/addgene:1791 ; RRID:Addgene_1791)
For your References section:Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP, Gygi SP, Sinclair DA, Alt FW, Greenberg ME. Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19. 10.1126/science.1094637 PubMed 14976264