The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo.
Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 44011 | pInducer10-mir-RUP-PheS | Lentiviral shRNA vector (pGIPz based) for inducible knockdown |
| 44012 | pInducer20 | Tet-inducible lentiviral vector for ORF expression |
| 44363 | pINDUCER11 (miR-RUG) | Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest |
| 46935 | pINDUCER12 (miR-RUL) | Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest |
| 46936 | pINDUCER13 (miR-LUP) | Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest |
| 46948 | pINDUCER21 (ORF-EG) | Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest |