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(Plasmid #46948)


Item Catalog # Description Quantity Price (USD)
Plasmid 46948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Dr. Richard Mulligan
  • Backbone size (bp) 4874
  • Modifications to backbone
    pINDUCER21 (ORF-UG) was made by digesting pINDUCER20 with NdeI and SphI. eGFP was PCR amplified with AgeI and SphI on the 5′ and 3′ ends, respectively, and then digested with AgeI and SphI. The vector and eGFP were ligated and sequence verified. pINDUCER20 (ORF-UN) was made by digesting TRE2 out of the pSLIK-Neo- TGmiR-Luc vector with BamHI and SpeI and blunt-end cloned into the backbone of SpeI and XhoI digested pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston) to generate the pHAGE-TRE vector. A fragment containing the gateway recipient cassette was cut out of the pHAGE-EF-DEST vector (G.H.) with SpeI and inserted into pHAGE-TRE to generate the pHAGE-TRE-DEST vector. The Ubc-rtTA3-IRES-Neo fragment was cut out of the pSLIK-Neo-TGmiR-Luc vector with PacI and ClaI and bluntend cloned into the backbone of SphI and ClaI digested pHAGE-TRE-DEST.
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    pInducer 21 should be grown in LB media containing 50ug/ml ampicillin + 30ug/ml Chloramphenicol. Easiest to grow this gene expression vector at 30 deg C. When transforming pInducer 21 (parent vector alone) into ecoli, use competant ecoli strain "ccdB" Invitrogen cat# A10460. Following replacement of ccdB surival + Chloramphenicol cassette w cDNA of interest, resultant plasmid is then ampicillin resistant only, and can be transformed into standard competant ecoli strains such as DH5alpha, Stbl3(minimizes LTR recombination), or NEB10
  • Copy number

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Information for Cloning Grade DNA (Catalog # ( Back to top )


Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.


  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDUCER21 (ORF-EG) was a gift from Stephen Elledge & Thomas Westbrook (Addgene plasmid # 46948 ; ; RRID:Addgene_46948)
  • For your References section:

    The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. 10.1073/pnas.1019736108 PubMed 21307310