Purpose(Empty Backbone) Tet-inducible lentiviral vector for ORF expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44012||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||44012-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 11791
Vector typeMammalian Expression, Lentiviral
Selectable markersNeomycin (select with G418)
/ Fusion Protein
- HA (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionspInducer20’s should be grown in LB with 50 ug/ml of ampicillin and 30 ug/ml of chloramphenicol.
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
pHAGE-TRE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Information for Cloning Grade DNA (Catalog # 44012-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pInducer20 was a gift from Stephen Elledge (Addgene plasmid # 44012 ; http://n2t.net/addgene:44012 ; RRID:Addgene_44012)
For your References section:The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, Elledge SJ. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 10.1073/pnas.1019736108. Epub 2011 Feb 9. 10.1073/pnas.1019736108 PubMed 21307310