164,909 results

1. pCAS

   Plasmid

   #206994

   Purpose

   Plasmid expressing both SpCas9 and SaCas9

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
   

   Use

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-gRNA

   Plasmid

   #206990

   Purpose

   A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   Donor plasmid-targeting SaCas9-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-mCherry-T2A-3xPuroR-Frame 1

   Plasmid

   #206992

   Purpose

   Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDNR-mCherry-T2A-3xPuroR-Frame 2

   Plasmid

   #206993

   Purpose

   Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sfp pet29b C-terminal His Tag

   Plasmid

   #75015

   Purpose

   Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

   Depositor

   Michael Burkart

   Article

   Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

   Insert

   Sfp
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-NPC1_STOP

   Plasmid

   #161461

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Johannes Stuttmann, Sylvestre Marillonnet

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pHAGE NFkB-TA-LUC-UBC-GFP-W

   Plasmid

   #49343

   Purpose

   pHAGE NFkB-TA-LUC-UBC-GFP-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   eGFP (eGFP )
   Luciferase
   NFkB consensus binding sequence
   TA
   UBC
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Ryota Uehara, Gohta Goshima

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_nV5-AMOTL1

    Plasmid

    #172998

    Purpose

    constitutive expression of V5-tagged AMOTL1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL1 (AMOTL1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

    Plasmid

    #234046

    Purpose

    expresses LaccID on the mammalian cell surface

    Depositor

    Alice Ting

    Article

    Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

    Insert

    LaccID-CD4TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-RNF126

    Plasmid

    #138643

    Purpose

    Bacterial expression of GST-RNF126 fusion protein

    Depositor

    Jane McGlade

    Article

    Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. elavl3:hArr-TEV;he1.1:YFP

    Plasmid

    #218855

    Purpose

    D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hArr
    

    Use

    Tags

    human Arrestin-Tobbaco Etch Virus protease fusion…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-Soma-jGCaMP8m (AAV1)

    Viral Prep

    #169257-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Promoter

    Tags

    None

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Human V5-IRF3-pcDNA3

    Plasmid

    #32713

    Depositor

    Saumen Sarkar

    Article

    Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid

    #111744

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q30 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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