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pSPgRNA Citations (92)

Originally described in: RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
PubMed Journal

Articles Citing pSPgRNA

Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Kabadi AM, Gersbach CA. Methods. 2014 Sep;69(2):188-97. doi: 10.1016/j.ymeth.2014.06.014. Epub 2014 Jul 8. PubMed
Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. 2014 Aug 13. pii: gku749. PubMed

Associated Plasmids

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. PubMed

Associated Plasmids

Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nihongaki Y, Kawano F, Nakajima T, Sato M. Nat Biotechnol. 2015 Jul;33(7):755-60. doi: 10.1038/nbt.3245. Epub 2015 Jun 15. PubMed
TALEN- and CRISPR/Cas9-Mediated Gene Editing in Human Pluripotent Stem Cells Using Lipid-Based Transfection. Hendriks WT, Jiang X, Daheron L, Cowan CA. Curr Protoc Stem Cell Biol. 2015 Aug 3;34:5B.3.1-5B.3.25. doi: 10.1002/9780470151808.sc05b03s34. PubMed
CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed
Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter. Ji H, Jiang Z, Lu P, Ma L, Li C, Pan H, Fu Z, Qu X, Wang P, Deng J, Yang X, Wang J, Zhu H. Mol Ther. 2016 Mar;24(3):508-21. doi: 10.1038/mt.2016.7. Epub 2016 Jan 18. PubMed
CRISPR-on system for the activation of the endogenous human INS gene. Gimenez CA, Ielpi M, Mutto A, Grosembacher L, Argibay P, Pereyra-Bonnet F. Gene Ther. 2016 Jun;23(6):543-7. doi: 10.1038/gt.2016.28. Epub 2016 Apr 4. PubMed
Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System. Brown A, Woods WS, Perez-Pinera P. ACS Synth Biol. 2016 Jul 15;5(7):582-8. doi: 10.1021/acssynbio.6b00056. Epub 2016 May 9. PubMed
Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Comte D, Karampetsou MP, Kis-Toth K, Yoshida N, Bradley SJ, Mizui M, Kono M, Solomon JR, Kyttaris VC, Tsokos GC. Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):9321-6. doi: 10.1073/pnas.1605081113. Epub 2016 Aug 1. PubMed
Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Garcia-Bloj B, Moses C, Sgro A, Plani-Lam J, Arooj M, Duffy C, Thiruvengadam S, Sorolla A, Rashwan R, Mancera RL, Leisewitz A, Swift-Scanlan T, Corvalan AH, Blancafort P. Oncotarget. 2016 Sep 13;7(37):60535-60554. doi: 10.18632/oncotarget.11142. PubMed
Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system. Bevacqua RJ, Fernandez-Martin R, Savy V, Canel NG, Gismondi MI, Kues WA, Carlson DF, Fahrenkrug SC, Niemann H, Taboga OA, Ferraris S, Salamone DF. Theriogenology. 2016 Nov;86(8):1886-1896.e1. doi: 10.1016/j.theriogenology.2016.06.010. Epub 2016 Jun 15. PubMed
Targeted Gene Activation Using RNA-Guided Nucleases. Brown A, Woods WS, Perez-Pinera P. Methods Mol Biol. 2017;1468:235-50. doi: 10.1007/978-1-4939-4035-6_16. PubMed
CRISPR/Cas9 editing of induced pluripotent stem cells for engineering inflammation-resistant tissues. Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Arthritis Rheumatol. 2016 Nov 3. doi: 10.1002/art.39982. PubMed
Orthogonal genetic regulation in human cells using chemically induced CRISPR/Cas9 activators. Bao Z, Jain S, Jaroenpuntaruk V, Zhao H. ACS Synth Biol. 2017 Jan 5. doi: 10.1021/acssynbio.6b00313. PubMed
NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. Awwad SW, Abu-Zhayia ER, Guttmann-Raviv N, Ayoub N. EMBO Rep. 2017 May;18(5):745-764. doi: 10.15252/embr.201643191. Epub 2017 Mar 23. PubMed
Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs. Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Stem Cell Reports. 2017 May 9;8(5):1202-1213. doi: 10.1016/j.stemcr.2017.03.022. Epub 2017 Apr 27. PubMed
Prosurvival long noncoding RNA PINCR regulates a subset of p53 targets in human colorectal cancer cells by binding to Matrin 3. Chaudhary R, Gryder B, Woods WS, Subramanian M, Jones MF, Li XL, Jenkins LM, Shabalina SA, Mo M, Dasso M, Yang Y, Wakefield LM, Zhu Y, Frier SM, Moriarity BS, Prasanth KV, Perez-Pinera P, Lal A. Elife. 2017 Jun 5;6. doi: 10.7554/eLife.23244. PubMed
CRISPR Epigenome Editing of AKAP150 in DRG Neurons Abolishes Degenerative IVD-Induced Neuronal Activation. Stover JD, Farhang N, Berrett KC, Gertz J, Lawrence B, Bowles RD. Mol Ther. 2017 Sep 6;25(9):2014-2027. doi: 10.1016/j.ymthe.2017.06.010. Epub 2017 Jul 1. PubMed
Identification and characterization of a FOXA2-regulated transcriptional enhancer at a type 2 diabetes intronic locus that controls GCKR expression in liver cells. Lopez Rodriguez M, Kaminska D, Lappalainen K, Pihlajamaki J, Kaikkonen MU, Laakso M. Genome Med. 2017 Jul 6;9(1):63. doi: 10.1186/s13073-017-0453-x. PubMed
Effect of ELOVL3 expression on bovine skeletal muscle-derived satellite cell differentiation. Liu D, Xu JH, Tong HL, Li SF, Yan YQ. Biochem Biophys Res Commun. 2017 Dec 2;493(4):1457-1463. doi: 10.1016/j.bbrc.2017.07.118. Epub 2017 Aug 3. PubMed
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
The activation of STIM1 mediates S-phase arrest and cell death in paraquat induced acute lung intoxication. Fan H, Huang H, Hu L, Zhu W, Yu Y, Lou J, Hu L, Chen F. Toxicol Lett. 2018 Aug;292:123-135. doi: 10.1016/j.toxlet.2018.04.030. Epub 2018 Apr 27. PubMed
Targeted Gene Knock Out Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1. Gapinske M, Tague N, Winter J, Underhill GH, Perez-Pinera P. Methods Mol Biol. 2018;1772:233-248. doi: 10.1007/978-1-4939-7795-6_13. PubMed
CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells. Marx N, Grunwald-Gruber C, Bydlinski N, Dhiman H, Ngoc Nguyen L, Klanert G, Borth N. Biotechnol J. 2018 Oct;13(10):e1700217. doi: 10.1002/biot.201700217. Epub 2018 Jun 11. PubMed
Enhanced Bacterial Immunity and Mammalian Genome Editing via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks. Clarke R, Heler R, MacDougall MS, Yeo NC, Chavez A, Regan M, Hanakahi L, Church GM, Marraffini LA, Merrill BJ. Mol Cell. 2018 Jul 5;71(1):42-55.e8. doi: 10.1016/j.molcel.2018.06.005. PubMed
Targeted Reactivation of FMR1 Transcription in Fragile X Syndrome Embryonic Stem Cells. Haenfler JM, Skariah G, Rodriguez CM, Monteiro da Rocha A, Parent JM, Smith GD, Todd PK. Front Mol Neurosci. 2018 Aug 15;11:282. doi: 10.3389/fnmol.2018.00282. eCollection 2018. PubMed
Disruption of the beta1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner. Mancini A, Xavier-Magalhaes A, Woods WS, Nguyen KT, Amen AM, Hayes JL, Fellmann C, Gapinske M, McKinney AM, Hong C, Jones LE, Walsh KM, Bell RJA, Doudna JA, Costa BM, Song JS, Perez-Pinera P, Costello JF. Cancer Cell. 2018 Sep 10;34(3):513-528.e8. doi: 10.1016/j.ccell.2018.08.003. PubMed
Podocan affects C2C12 myogenic differentiation by enhancing Wnt/beta-catenin signaling. Liu D, Li S, Cui Y, Tong H, Li S, Yan Y. J Cell Physiol. 2019 Jul;234(7):11130-11139. doi: 10.1002/jcp.27763. Epub 2019 Jan 16. PubMed
High-Dose Paraquat Induces Human Bronchial 16HBE Cell Death and Aggravates Acute Lung Intoxication in Mice by Regulating Keap1/p65/Nrf2 Signal Pathway. Yao J, Zhang J, Tai W, Deng S, Li T, Wu W, Pu L, Fan D, Lei W, Zhang T, Dong Z. Inflammation. 2019 Apr;42(2):471-484. doi: 10.1007/s10753-018-00956-1. PubMed
High-glucose induces retinal pigment epithelium mitochondrial pathways of apoptosis and inhibits mitophagy by regulating ROS/PINK1/Parkin signal pathway. Zhang Y, Xi X, Mei Y, Zhao X, Zhou L, Ma M, Liu S, Zha X, Yang Y. Biomed Pharmacother. 2019 Mar;111:1315-1325. doi: 10.1016/j.biopha.2019.01.034. Epub 2019 Jan 15. PubMed
Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration. Brown A, Winter J, Gapinske M, Tague N, Woods WS, Perez-Pinera P. Nucleic Acids Res. 2019 Jul 9;47(12):e67. doi: 10.1093/nar/gkz210. PubMed
Regulation of alternative splicing by p300-mediated acetylation of splicing factors. Siam A, Baker M, Amit L, Regev G, Rabner A, Najar RA, Bentata M, Dahan S, Cohen K, Araten S, Nevo Y, Kay G, Mandel-Gutfreund Y, Salton M. RNA. 2019 Jul;25(7):813-824. doi: 10.1261/rna.069856.118. Epub 2019 Apr 15. PubMed
Increasing the specificity of CRISPR systems with engineered RNA secondary structures. Kocak DD, Josephs EA, Bhandarkar V, Adkar SS, Kwon JB, Gersbach CA. Nat Biotechnol. 2019 Jun;37(6):657-666. doi: 10.1038/s41587-019-0095-1. Epub 2019 Apr 15. PubMed
GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. Li S, Fu Y, Pang Y, Tong H, Li S, Yan Y. J Cell Physiol. 2019 Nov;234(11):21211-21223. doi: 10.1002/jcp.28727. Epub 2019 Apr 25. PubMed
Multiplex Epigenome Editing of Dorsal Root Ganglion Neuron Receptors Abolishes Redundant Interleukin 6, Tumor Necrosis Factor Alpha, and Interleukin 1beta Signaling by the Degenerative Intervertebral Disc. Stover JD, Farhang N, Lawrence B, Bowles RD. Hum Gene Ther. 2019 Jun 11. doi: 10.1089/hum.2019.032. PubMed
Intracellular Ca(2+) Homeostasis and Nuclear Export Mediate Exit from Naive Pluripotency. MacDougall MS, Clarke R, Merrill BJ. Cell Stem Cell. 2019 Aug 1;25(2):210-224.e6. doi: 10.1016/j.stem.2019.04.015. Epub 2019 May 16. PubMed
A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism. Diquigiovanni C, Bergamini C, Diaz R, Liparulo I, Bianco F, Masin L, Baldassarro VA, Rizzardi N, Tranchina A, Buscherini F, Wischmeijer A, Pippucci T, Scarano E, Cordelli DM, Fato R, Seri M, Paracchini S, Bonora E. FASEB J. 2019 Oct;33(10):11284-11302. doi: 10.1096/fj.201802722R. Epub 2019 Aug 7. PubMed
Identification of a pituitary ERalpha-activated enhancer triggering the expression of Nr5a1, the earliest gonadotrope lineage-specific transcription factor. Pacini V, Petit F, Querat B, Laverriere JN, Cohen-Tannoudji J, L'hote D. Epigenetics Chromatin. 2019 Aug 7;12(1):48. doi: 10.1186/s13072-019-0291-8. PubMed
Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state. Menges J, Cremanns M, Steenpass L. Stem Cell Res. 2019 Aug;39:101517. doi: 10.1016/j.scr.2019.101517. Epub 2019 Jul 29. PubMed
Gene Editing Preserves Visual Functions in a Mouse Model of Retinal Degeneration. Vagni P, Perlini LE, Chenais NAL, Marchetti T, Parrini M, Contestabile A, Cancedda L, Ghezzi D. Front Neurosci. 2019 Sep 10;13:945. doi: 10.3389/fnins.2019.00945. eCollection 2019. PubMed
Coordinate regulation of ELF5 and EHF at the chr11p13 CF modifier region. Swahn H, Sabith Ebron J, Lamar KM, Yin S, Kerschner JL, NandyMazumdar M, Coppola C, Mendenhall EM, Leir SH, Harris A. J Cell Mol Med. 2019 Nov;23(11):7726-7740. doi: 10.1111/jcmm.14646. Epub 2019 Sep 26. PubMed
Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient. Dal-Pra S, Hodgkinson CP, Dzau VJ. PLoS One. 2019 Oct 17;14(10):e0223842. doi: 10.1371/journal.pone.0223842. eCollection 2019. PubMed
Hypoxia drives glucose transporter 3 expression through HIF-mediated induction of the long non-coding RNA NICI. Lauer V, Grampp S, Platt J, Lafleur V, Lombardi O, Choudhry H, Kranz F, Hartmann A, Wullich B, Yamamoto A, Coleman ML, Ratcliffe PJ, Mole DR, Schodel J. J Biol Chem. 2019 Nov 5. pii: RA119.009827. doi: 10.1074/jbc.RA119.009827. PubMed
The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Katsuyama E, Suarez-Fueyo A, Bradley SJ, Mizui M, Marin AV, Mulki L, Krishfield S, Malavasi F, Yoon J, Sui SJH, Kyttaris VC, Tsokos GC. Cell Rep. 2020 Jan 7;30(1):112-123.e4. doi: 10.1016/j.celrep.2019.12.014. PubMed
The construction of a new oncolytic herpes simplex virus expressing murine interleukin-15 with gene-editing technology. Cai L, Hu H, Duan H, Li Y, Zou Z, Luo K, Zhang Z, Yang J, Jin J, Chen Y, Ke Z, Fang Z, Liu Q, Hong X, Hu S, Liu B. J Med Virol. 2020 Jan 29. doi: 10.1002/jmv.25691. PubMed
TCP11L2 promotes bovine skeletal muscle-derived satellite cell migration and differentiation via FMNL2. Li S, Wang Z, Tong H, Li S, Yan Y. J Cell Physiol. 2020 Oct;235(10):7183-7193. doi: 10.1002/jcp.29617. Epub 2020 Feb 4. PubMed
Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing. Artegiani B, Hendriks D, Beumer J, Kok R, Zheng X, Joore I, Chuva de Sousa Lopes S, van Zon J, Tans S, Clevers H. Nat Cell Biol. 2020 Mar;22(3):321-331. doi: 10.1038/s41556-020-0472-5. Epub 2020 Mar 2. PubMed
WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-beta signalling pathway. Zhang C, Zhang Y, Zhang W, Tong H, Li S, Yan Y. Mol Cell Biochem. 2020 Jul;470(1-2):215-227. doi: 10.1007/s11010-020-03763-1. Epub 2020 May 27. PubMed
Baculovirus Transduction in Mammalian Cells Is Affected by the Production of Type I and III Interferons, Which Is Mediated Mainly by the cGAS-STING Pathway. Amalfi S, Molina GN, Bevacqua RJ, Lopez MG, Taboga O, Alfonso V. J Virol. 2020 Oct 14;94(21). pii: JVI.01555-20. doi: 10.1128/JVI.01555-20. Print 2020 Oct 14. PubMed
Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems. Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ. Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671. PubMed

Associated Plasmids

Restoration of visual function in adult mice with an inherited retinal disease via adenine base editing. Suh S, Choi EH, Leinonen H, Foik AT, Newby GA, Yeh WH, Dong Z, Kiser PD, Lyon DC, Liu DR, Palczewski K. Nat Biomed Eng. 2021 Feb;5(2):169-178. doi: 10.1038/s41551-020-00632-6. Epub 2020 Oct 19. PubMed
Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation. Kubo N, Ishii H, Xiong X, Bianco S, Meitinger F, Hu R, Hocker JD, Conte M, Gorkin D, Yu M, Li B, Dixon JR, Hu M, Nicodemi M, Zhao H, Ren B. Nat Struct Mol Biol. 2021 Feb;28(2):152-161. doi: 10.1038/s41594-020-00539-5. Epub 2021 Jan 4. PubMed
Regulation of MYB by distal enhancer elements in human myeloid leukemia. Li M, Jiang P, Cheng K, Zhang Z, Lan S, Li X, Zhao L, Wang Y, Wang X, Chen J, Ji T, Han B, Zhang J. Cell Death Dis. 2021 Feb 26;12(2):223. doi: 10.1038/s41419-021-03515-z. PubMed
Independent domains for recruitment of PRC1 and PRC2 by human XIST. Dixon-McDougall T, Brown CJ. PLoS Genet. 2021 Mar 22;17(3):e1009123. doi: 10.1371/journal.pgen.1009123. eCollection 2021 Mar. PubMed
Precise Regulation of Cas9-Mediated Genome Engineering by Anti-CRISPR-Based Inducible CRISPR Controllers. Jain S, Xun G, Abesteh S, Ho S, Lingamaneni M, Martin TA, Tasan I, Yang C, Zhao H. ACS Synth Biol. 2021 Jun 18;10(6):1320-1327. doi: 10.1021/acssynbio.0c00548. Epub 2021 May 18. PubMed
Optical Control of Genome Editing by Photoactivatable Cas9. Otabe T, Nihongaki Y, Sato M. Methods Mol Biol. 2021;2312:225-233. doi: 10.1007/978-1-0716-1441-9_13. PubMed
VEGFA's distal enhancer regulates its alternative splicing in CML. Dahan S, Sharma A, Cohen K, Baker M, Taqatqa N, Bentata M, Engal E, Siam A, Kay G, Drier Y, Elias S, Salton M. NAR Cancer. 2021 Jul 13;3(3):zcab029. doi: 10.1093/narcan/zcab029. eCollection 2021 Sep. PubMed
Human sensorimotor organoids derived from healthy and amyotrophic lateral sclerosis stem cells form neuromuscular junctions. Pereira JD, DuBreuil DM, Devlin AC, Held A, Sapir Y, Berezovski E, Hawrot J, Dorfman K, Chander V, Wainger BJ. Nat Commun. 2021 Aug 6;12(1):4744. doi: 10.1038/s41467-021-24776-4. PubMed
Defective Lipid Droplet-Lysosome Interaction Causes Fatty Liver Disease as Evidenced by Human Mutations in TMEM199 and CCDC115. Larsen LE, van den Boogert MAW, Rios-Ocampo WA, Jansen JC, Conlon D, Chong PLE, Levels JHM, Eilers RE, Sachdev VV, Zelcer N, Raabe T, He M, Hand NJ, Drenth JPH, Rader DJ, Stroes ESG, Lefeber DJ, Jonker JW, Holleboom AG. Cell Mol Gastroenterol Hepatol. 2022;13(2):583-597. doi: 10.1016/j.jcmgh.2021.09.013. Epub 2021 Oct 7. PubMed
KIAA0319 influences cilia length, cell migration and mechanical cell-substrate interaction. Diaz R, Kronenberg NM, Martinelli A, Liehm P, Riches AC, Gather MC, Paracchini S. Sci Rep. 2022 Jan 14;12(1):722. doi: 10.1038/s41598-021-04539-3. PubMed
Shear stress switches the association of endothelial enhancers from ETV/ETS to KLF transcription factor binding sites. Tsaryk R, Yucel N, Leonard EV, Diaz N, Bondareva O, Odenthal-Schnittler M, Arany Z, Vaquerizas JM, Schnittler H, Siekmann AF. Sci Rep. 2022 Mar 21;12(1):4795. doi: 10.1038/s41598-022-08645-8. PubMed
Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1-induced inflammation. Pferdehirt L, Damato AR, Dudek M, Meng QJ, Herzog ED, Guilak F. Sci Adv. 2022 May 27;8(21):eabj8892. doi: 10.1126/sciadv.abj8892. Epub 2022 May 25. PubMed
Systematic HIV-1 promoter targeting with CRISPR/dCas9-VPR reveals optimal region for activation of the latent provirus. Klinnert S, Chemnitzer A, Rusert P, Metzner KJ. J Gen Virol. 2022 Jun;103(6). doi: 10.1099/jgv.0.001754. PubMed
A red light-responsive photoswitch for deep tissue optogenetics. Kuwasaki Y, Suzuki K, Yu G, Yamamoto S, Otabe T, Kakihara Y, Nishiwaki M, Miyake K, Fushimi K, Bekdash R, Shimizu Y, Narikawa R, Nakajima T, Yazawa M, Sato M. Nat Biotechnol. 2022 Jun 13. pii: 10.1038/s41587-022-01351-w. doi: 10.1038/s41587-022-01351-w. PubMed
The Activation of Protamine 1 Using Epigenome Editing Decreases the Proliferation of Tumorigenic Cells. Namous H, Braz CU, Wang Y, Khatib H. Front Genome Ed. 2022 Jun 16;4:844904. doi: 10.3389/fgeed.2022.844904. eCollection 2022. PubMed
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed
Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells. Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB. ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 10.1021/acssynbio.2c00156. Epub 2022 Sep 26. PubMed

Associated Plasmids

In silico design of CMV promoter binding oligonucleotides and their impact on inhibition of gene expression in Chinese hamster ovary cells. Hussein MK, Papez M, Dhiman H, Baumann M, Galosy S, Borth N. J Biotechnol. 2022 Nov 20;359:185-193. doi: 10.1016/j.jbiotec.2022.10.003. Epub 2022 Oct 12. PubMed
Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells. Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T. Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7. PubMed

Associated Plasmids

Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models. Beumer J, Bauza-Martinez J, Veth TS, Geurts V, Boot C, Gilliam-Vigh H, Poulsen SS, Knop FK, Wu W, Clevers H. Proc Natl Acad Sci U S A. 2022 Nov 16;119(46):e2212057119. doi: 10.1073/pnas.2212057119. Epub 2022 Nov 7. PubMed
CHD7 regulates otic lineage specification and hair cell differentiation in human inner ear organoids. Nie J, Ueda Y, Solivais AJ, Hashino E. Nat Commun. 2022 Nov 17;13(1):7053. doi: 10.1038/s41467-022-34759-8. PubMed
XLID syndrome gene Med12 promotes Ig isotype switching through chromatin modification and enhancer RNA regulation. Haque F, Honjo T, Begum NA. Sci Adv. 2022 Nov 25;8(47):eadd1466. doi: 10.1126/sciadv.add1466. Epub 2022 Nov 25. PubMed
Non-synonymous, synonymous, and non-coding nucleotide variants contribute to recurrently altered biological processes during retinoblastoma progression. Stachelek K, Harutyunyan N, Lee S, Beck A, Kim J, Xu L, Berry JL, Nagiel A, Reynolds CP, Murphree AL, Lee TC, Aparicio JG, Cobrinik D. Genes Chromosomes Cancer. 2023 May;62(5):275-289. doi: 10.1002/gcc.23120. Epub 2023 Jan 5. PubMed
De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs. Motosugi N, Sugiyama A, Okada C, Otomo A, Umezawa A, Akutsu H, Hadano S, Fukuda A. Cell Rep Methods. 2022 Nov 29;2(12):100352. doi: 10.1016/j.crmeth.2022.100352. eCollection 2022 Dec 19. PubMed
ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. Lin G, Qi H, Guo X, Wang W, Zhang M, Gao X. J Nutr Biochem. 2023 Apr;114:109274. doi: 10.1016/j.jnutbio.2023.109274. Epub 2023 Jan 18. PubMed
TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation. Horvath RM, Dahabieh M, Malcolm T, Sadowski I. Commun Biol. 2023 Jan 23;6(1):86. doi: 10.1038/s42003-023-04484-z. PubMed
Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression. Wu J, Larreategui-Aparicio A, Lambers MLA, Bodor DL, Klaasen SJ, Tollenaar E, de Ruijter-Villani M, Kops GJPL. Curr Biol. 2023 Mar 13;33(5):912-925.e6. doi: 10.1016/j.cub.2023.01.010. Epub 2023 Jan 30. PubMed
Dual-AAV split prime editor corrects the mutation and phenotype in mice with inherited retinal degeneration. She K, Liu Y, Zhao Q, Jin X, Yang Y, Su J, Li R, Song L, Xiao J, Yao S, Lu F, Wei Y, Yang Y. Signal Transduct Target Ther. 2023 Feb 6;8(1):57. doi: 10.1038/s41392-022-01234-1. PubMed
ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells. Guo X, Qi H, Lin G, Yu J, Zhang M, Gao X. Cell Biol Int. 2023 Jun;47(6):1106-1117. doi: 10.1002/cbin.12003. Epub 2023 Feb 14. PubMed
In vivo adenine base editing corrects newborn murine model of Hurler syndrome. Su J, Jin X, She K, Liu Y, Song L, Zhao Q, Xiao J, Li R, Deng H, Lu F, Yang Y. Mol Biomed. 2023 Feb 23;4(1):6. doi: 10.1186/s43556-023-00120-8. PubMed
Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis. Hendriks D, Brouwers JF, Hamer K, Geurts MH, Luciana L, Massalini S, Lopez-Iglesias C, Peters PJ, Rodriguez-Colman MJ, Chuva de Sousa Lopes S, Artegiani B, Clevers H. Nat Biotechnol. 2023 Feb 23. doi: 10.1038/s41587-023-01680-4. PubMed
Distinct roles of Bdnf I and Bdnf IV transcript variant expression in hippocampal neurons. Bach SV, Bauman AJ, Hosein D, Tuscher JJ, Ianov L, Greathouse KM, Henderson BW, Herskowitz JH, Martinowich K, Day JJ. bioRxiv. 2023 Apr 5:2023.04.05.535694. doi: 10.1101/2023.04.05.535694. Preprint. PubMed
Organoid models of fibrolamellar carcinoma mutations reveal hepatocyte transdifferentiation through cooperative BAP1 and PRKAR2A loss. Ruland L, Andreatta F, Massalini S, Chuva de Sousa Lopes S, Clevers H, Hendriks D, Artegiani B. Nat Commun. 2023 May 3;14(1):2377. doi: 10.1038/s41467-023-37951-6. PubMed
Isoforms of the TAL1 transcription factor have different roles in hematopoiesis and cell growth. Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, Plaschkes I, Kay G, Drier Y, Berger M, Salton M. PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 10.1371/journal.pbio.3002175. PubMed

Associated Plasmids

Upstream Stimulatory Factors Regulate HIV-1 Latency and Are Required for Robust T Cell Activation. Horvath RM, Sadowski I. Viruses. 2023 Jun 28;15(7):1470. doi: 10.3390/v15071470. PubMed
Targeted shock-and-kill HIV-1 gene therapy approach combining CRISPR activation, suicide gene tBid and retargeted adenovirus delivery. Klinnert S, Schenkel CD, Freitag PC, Gunthard HF, Pluckthun A, Metzner KJ. Gene Ther. 2023 Aug 9. doi: 10.1038/s41434-023-00413-1. PubMed
The Neurod1/4-Ntrk3-Src pathway regulates gonadotrope cell adhesion and motility. Le Cicle C, Pacini V, Rama N, Tauszig-Delamasure S, Airaud E, Petit F, de Beco S, Cohen-Tannoudji J, L'hote D. Cell Death Discov. 2023 Sep 1;9(1):327. doi: 10.1038/s41420-023-01615-7. PubMed
CDK8 inhibitors antagonize HIV-1 reactivation and promote provirus latency in T cells. Horvath RM, Brumme ZL, Sadowski I. J Virol. 2023 Sep 28;97(9):e0092323. doi: 10.1128/jvi.00923-23. Epub 2023 Sep 6. PubMed
Hypoxic regulation of hypoxia inducible factor 1 alpha via antisense transcription. Downes N, Niskanen H, Tomas Bosch V, Taipale M, Godiwala M, Vaananen MA, Turunen TA, Aavik E, Laham-Karam N, Yla-Herttuala S, Kaikkonen MU. J Biol Chem. 2023 Nov;299(11):105291. doi: 10.1016/j.jbc.2023.105291. Epub 2023 Sep 23. PubMed
A dual role of RBM42 in modulating splicing and translation of CDKN1A/p21 during DNA damage response. Ben-Oz BM, Machour FE, Nicola M, Argoetti A, Polyak G, Hanna R, Kleifeld O, Mandel-Gutfreund Y, Ayoub N. Nat Commun. 2023 Nov 22;14(1):7628. doi: 10.1038/s41467-023-43495-6. PubMed
ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis. Namous H, Strillacci MG, Braz CU, Shanmuganayagam D, Krueger C, Peppas A, Soffregen WC, Reed J, Granada JF, Khatib H. Atheroscler Plus. 2023 Nov 15;54:30-41. doi: 10.1016/j.athplu.2023.11.001. eCollection 2023 Dec. PubMed

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