pCMV-PE2-p53DD
(Plasmid #196582)

• Purpose: Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD
• Depositing Lab: Ting Zhou
• Publication: Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7.

Backbone

• Vector backbone: pCMV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2-P2A-mp53DD
• Alt name: SV40_bpNLS-SpCas9(H840A)-SGGSx2-XTEN16-SGGSx2-MMLV_RT(D200N, T306K, W313F, T330P, L603W)-SV40_bpNLS
• Alt name: Trp53 carboxy-terminal dominant-negative fragment
• Species: M. musculus (mouse), Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
• Insert Size (bp): 6716
• Mutation: Δ40-903
• GenBank ID: NM_011640
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • pCMV-PE2-p53DD (uploaded to Addgene).gbk
• A portion of this plasmid was derived from a plasmid made by: PE2 is from Addgene Plasmid #132775; p53DD is from Addgene Plasmid #41856.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry