Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61357||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerInvitrogen; Life Technologies
- Total vector size (bp) 11475
Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Growth instructionsMay require longer growth times.
Copy numberLow Copy
Gene/Insert nameS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
SpeciesH. sapiens (human), Synthetic; S. pyogenes
Insert Size (bp)6138
MutationD10A; H840A in S.pyogenes Cas9
GenBank IDWP_010922251.1 NP_001420.2
Entrez GeneEP300 (a.k.a. KAT3B, RSTS2, p300)
- Promoter CMV
/ Fusion Proteins
- Flag (N terminal on insert)
- SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer CMV F
- 3′ sequencing primer Bgh R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-dCas9-p300 Core was a gift from Charles Gersbach (Addgene plasmid # 61357)
For your References section:Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. 10.1038/nbt.3199 PubMed 25849900
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.