PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47108||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||47108-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3223
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Alt nameS. Pyogenes guide RNA
- Promoter U6
- Cloning method Unknown
- 5′ sequencing primer hU6-F
- 3′ sequencing primer T3 (Common Sequencing Primers)
For more information on Gersbach Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Gersbach/
Information for Cloning Grade DNA (Catalog # 47108-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSPgRNA was a gift from Charles Gersbach (Addgene plasmid # 47108 ; http://n2t.net/addgene:47108 ; RRID:Addgene_47108)
For your References section:RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. 10.1038/nmeth.2600 PubMed 23892895