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PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells
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Depositing Lab
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Citations
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZDonor
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Backbone manufacturerSigma
- Total vector size (bp) 3223
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPgRNA
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Alt nameS. Pyogenes guide RNA
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SpeciesSynthetic
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer hU6-F
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPgRNA was a gift from Charles Gersbach (Addgene plasmid # 47108 ; http://n2t.net/addgene:47108 ; RRID:Addgene_47108) -
For your References section:
RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. 10.1038/nmeth.2600 PubMed 23892895
Map uploaded by the depositor.
