|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63798||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.3 TOPO
Vector typeMammalian Expression, CRISPR, Synthetic Biology
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)5799
- Promoter CMV
/ Fusion Protein
- VPR (VP64-p65-Rta) (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.
This plasmid contains a human codon optimized form of cas9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SP-dCas9-VPR was a gift from George Church (Addgene plasmid # 63798)
For your References section:Highly efficient Cas9-mediated transcriptional programming. Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. 10.1038/nmeth.3312 PubMed 25730490