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Highly efficient Cas9-mediated transcriptional programming.
Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63798SP-dCas9-VPRSP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector
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63799M_ST1n_VPRST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
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63800PB-TRE-dCas9-VPRSP-dCas9-VPR with doxycycline-inducible expression
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63801pAG414GPD-dCas9-VPRpAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector
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63802pAWG-dCas9-VPRSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector
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64046pSB700Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
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Antibodies from Article