Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Highly efficient Cas9-mediated transcriptional programming.
Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63798SP-dCas9-VPRSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
Loading...
63799M_ST1n_VPRST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
Loading...
63800PB-TRE-dCas9-VPRSP-dCas9-VPR with doxycycline-inducible expression
Loading...
63801pAG414GPD-dCas9-VPRpAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector
Loading...
63802pAWG-dCas9-VPRSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector
Loading...
64046pSB700Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
Loading...