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pAG414GPD-dCas9-VPR
(Plasmid #63801)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63801 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAG414GPD
  • Vector type
    Yeast Expression, CRISPR, Synthetic Biology
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-VPR
  • Alt name
    VP64-p65-Rta
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5733
  • Promoter GPD
  • Tag / Fusion Protein
    • VPR (VP64-p65-Rta) (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG414GPD-dCas9-VPR was a gift from George Church (Addgene plasmid # 63801)
  • For your References section:

    Highly efficient Cas9-mediated transcriptional programming. Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. 10.1038/nmeth.3312 PubMed 25730490