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pcDNA-dCas9-p300 Core Citations (28)

Plasmid Article: Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
PubMed Journal

Articles Citing pcDNA-dCas9-p300 Core

CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Comparison of Cas9 activators in multiple species. Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed

Associated Plasmids

Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Garcia-Bloj B, Moses C, Sgro A, Plani-Lam J, Arooj M, Duffy C, Thiruvengadam S, Sorolla A, Rashwan R, Mancera RL, Leisewitz A, Swift-Scanlan T, Corvalan AH, Blancafort P. Oncotarget. 2016 Sep 13;7(37):60535-60554. doi: 10.18632/oncotarget.11142. PubMed
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. Cell. 2016 Sep 22;167(1):219-232.e14. doi: 10.1016/j.cell.2016.09.006. PubMed
Targeted Gene Activation Using RNA-Guided Nucleases. Brown A, Woods WS, Perez-Pinera P. Methods Mol Biol. 2017;1468:235-50. doi: 10.1007/978-1-4939-4035-6_16. PubMed
CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. PubMed

Associated Plasmids

Ethanol Exposure Regulates Gabra1 Expression via Histone Deacetylation at the Promoter in Cultured Cortical Neurons. Bohnsack JP, Patel VK, Morrow AL. J Pharmacol Exp Ther. 2017 Oct;363(1):1-11. doi: 10.1124/jpet.117.242446. Epub 2017 Aug 10. PubMed
CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency. Liu P, Chen M, Liu Y, Qi LS, Ding S. Cell Stem Cell. 2018 Feb 1;22(2):252-261.e4. doi: 10.1016/j.stem.2017.12.001. Epub 2018 Jan 18. PubMed
Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells. Gao D, Liang FS. Methods Mol Biol. 2018;1767:429-445. doi: 10.1007/978-1-4939-7774-1_24. PubMed
A novel enhancer regulates MGMT expression and promotes temozolomide resistance in glioblastoma. Chen X, Zhang M, Gan H, Wang H, Lee JH, Fang D, Kitange GJ, He L, Hu Z, Parney IF, Meyer FB, Giannini C, Sarkaria JN, Zhang Z. Nat Commun. 2018 Jul 27;9(1):2949. doi: 10.1038/s41467-018-05373-4. PubMed
Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells. Kuscu C, Mammadov R, Czikora A, Unlu H, Tufan T, Fischer NL, Arslan S, Bekiranov S, Kanemaki M, Adli M. J Mol Biol. 2019 Jan 4;431(1):111-121. doi: 10.1016/j.jmb.2018.08.001. Epub 2018 Aug 9. PubMed
Super-enhancers maintain renin-expressing cell identity and memory to preserve multi-system homeostasis. Martinez MF, Medrano S, Brown EA, Tufan T, Shang S, Bertoncello N, Guessoum O, Adli M, Belyea BC, Sequeira-Lopez MLS, Gomez RA. J Clin Invest. 2018 Nov 1;128(11):4787-4803. doi: 10.1172/JCI121361. Epub 2018 Oct 2. PubMed
Rationally-engineered reproductive barriers using CRISPR & CRISPRa: an evaluation of the synthetic species concept in Drosophila melanogaster. Waters AJ, Capriotti P, Gaboriau DCA, Papathanos PA, Windbichler N. Sci Rep. 2018 Sep 3;8(1):13125. doi: 10.1038/s41598-018-31433-2. PubMed
Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed

Associated Plasmids

CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Hsu MN, Liao HT, Truong VA, Huang KL, Yu FJ, Chen HH, Nguyen TKN, Makarevich P, Parfyonova Y, Hu YC. Theranostics. 2019 Aug 14;9(21):6099-6111. doi: 10.7150/thno.36790. eCollection 2019. PubMed
Sufficiency analysis of estrogen responsive enhancers using synthetic activators. Ginley-Hidinger M, Carleton JB, Rodriguez AC, Berrett KC, Gertz J. Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900497. doi: 10.26508/lsa.201900497. Print 2019 Oct. PubMed
ATM and ATR Expression Potentiates HBV Replication and Contributes to Reactivation of HBV Infection upon DNA Damage. Kostyusheva A, Brezgin S, Bayurova E, Gordeychuk I, Isaguliants M, Goptar I, Urusov F, Nikiforova A, Volchkova E, Kostyushev D, Chulanov V. Viruses. 2019 Oct 31;11(11). pii: v11110997. doi: 10.3390/v11110997. PubMed
Epigenetic therapy of novel tumour suppressor ZAR1 and its cancer biomarker function. Deutschmeyer V, Breuer J, Walesch SK, Sokol AM, Graumann J, Bartkuhn M, Boettger T, Rossbach O, Richter AM. Clin Epigenetics. 2019 Dec 4;11(1):182. doi: 10.1186/s13148-019-0774-2. PubMed
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J. Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5. PubMed

Associated Plasmids

Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9. Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J. Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. PubMed

Associated Plasmids

Noncoding Variants Connect Enhancer Dysregulation with Nuclear Receptor Signaling in Hematopoietic Malignancies. Li K, Zhang Y, Liu X, Liu Y, Gu Z, Cao H, Dickerson KE, Chen M, Chen W, Shao Z, Ni M, Xu J. Cancer Discov. 2020 May;10(5):724-745. doi: 10.1158/2159-8290.CD-19-1128. Epub 2020 Mar 18. PubMed
Reversible promoter methylation determines fluctuating expression of acute phase proteins. Zhang SC, Wang MY, Feng JR, Chang Y, Ji SR, Wu Y. Elife. 2020 Mar 30;9. pii: 51317. doi: 10.7554/eLife.51317. PubMed
KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Lipinski M, Munoz-Viana R, Del Blanco B, Marquez-Galera A, Medrano-Relinque J, Carames JM, Szczepankiewicz AA, Fernandez-Albert J, Navarron CM, Olivares R, Wilczynski GM, Canals S, Lopez-Atalaya JP, Barco A. Nat Commun. 2020 May 22;11(1):2588. doi: 10.1038/s41467-020-16246-0. PubMed
Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems. Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ. Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671. PubMed

Associated Plasmids

Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases. Brezgin S, Kostyusheva A, Ponomareva N, Volia V, Goptar I, Nikiforova A, Shilovskiy I, Smirnov V, Kostyushev D, Chulanov V. Int J Mol Sci. 2020 Sep 18;21(18). pii: ijms21186865. doi: 10.3390/ijms21186865. PubMed
Profiling of H3K27Ac Reveals the Influence of Asthma on the Epigenome of the Airway Epithelium. McErlean P, Kelly A, Dhariwal J, Kirtland M, Watson J, Ranz I, Smith J, Saxena A, Cousins DJ, Van Oosterhout A, Solari R, Edwards MR, Johnston SL, Lavender P. Front Genet. 2020 Dec 10;11:585746. doi: 10.3389/fgene.2020.585746. eCollection 2020. PubMed
Lupus susceptibility region containing CDKN1B rs34330 mechanistically influences expression and function of multiple target genes, also linked to proliferation and apoptosis. Singh B, Maiti GP, Zhou X, Fazel-Najafabadi M, Bae SC, Sun C, Terao C, Okada Y, Chua KH, Kochi Y, Guthridge JM, Zhang H, Weirauch M, James JA, Harley JB, Varshney GK, Looger LL, Nath SK. Arthritis Rheumatol. 2021 May 13. doi: 10.1002/art.41799. PubMed

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