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lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP
(Plasmid #159086)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 159086 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang Addgene plasmid #52961
  • Backbone size w/o insert (bp) 6789
  • Total vector size (bp) 13374
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    6585
  • Promoter hSYN, U6
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • EGFP

Cloning Information

Resource Information

Depositor Comments

CRISPR crRNA and acRNA are empty - use BbsI to insert desired crRNA and Esp3I to insert desired acRNA. bioRxiv: https://www.biorxiv.org/content/10.1101/270967v3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP was a gift from Jeremy Day (Addgene plasmid # 159086 ; http://n2t.net/addgene:159086 ; RRID:Addgene_159086)
  • For your References section:

    Enhancer RNAs predict enhancer-gene regulatory links and are critical for enhancer function in neuronal systems. Carullo NVN, Phillips Iii RA, Simon RC, Soto SAR, Hinds JE, Salisbury AJ, Revanna JS, Bunner KD, Ianov L, Sultan FA, Savell KE, Gersbach CA, Day JJ. Nucleic Acids Res. 2020 Aug 18. pii: 5893972. doi: 10.1093/nar/gkaa671. 10.1093/nar/gkaa671 PubMed 32810208