Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing.
Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB
Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed Article
Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB
Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121686 | HB401: pMVP (L1-L4) CMV promoter | pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly |
| 121687 | IF101: pMVP (L1-L4) EF1a promoter | pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly |
| 121688 | HB501: pMVP (L1-L4) RIP | pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly |
| 121689 | IG001: pMVP (L1-L4) TBG promoter | pMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly |
| 121690 | II701: pMVP (L1-L4) cTnT promoter | pMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assembly |
| 121691 | IG603: pMVP (L1-L4) TRE promoter | pMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly |
| 121692 | IG301: pMVP (L1-R5) CMV promoter | pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly |
| 121693 | IY704: pMVP (L1-R5) EF1a promoter | pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly |
| 121694 | IG401: pMVP (L1-R5) RIP | pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly |
| 121695 | LF601: pMVP (L1-R5) TBG promoter | pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly |
| 121696 | LF801: pMVP (L1-R5) cTnT promoter | pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly |
| 121697 | IG703: pMVP (L1-R5) TRE promoter | pMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly |
| 121698 | HE401: pMVP (L1-R5) shRNA | pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly |
| 121699 | IH001: pMVP (L5-L4) HA epitope tag | pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121700 | IH201: pMVP (L5-L4) 3x HA epitope tag | pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121701 | IG901: pMVP (L5-L4) FLAG epitope tag | pMVP L5-L4 entry plasmid, contains FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121702 | IO801: pMVP (L5-L4) 3x FLAG epitope tag | pMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121703 | IH101: pMVP (L5-L4) OLLAS epitope tag | pMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121704 | IX104: pMVP (L5-L4) myc epitope tag | pMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121705 | IX203: pMVP (L5-L4) V5 epitope tag | pMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121706 | KG703: pMVP (L5-L4) 6x His epitope tag | pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest. |
| 121707 | KU701: pMVP (L5-L4) eGFP | pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest. |
| 121708 | KP201: pMVP (L5-L4) eGFP-P2A | pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest. |
| 121709 | KU801: pMVP (L5-L4) mCherry | pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest. |
| 121710 | KP301: pMVP (L5-L4) mCherry-P2A | pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest. |
| 121711 | KU901: pMVP (L5-L4) eCFP | pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest. |
| 121712 | KP401: pMVP (L5-L4) eCFP-P2A | pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest. |
| 121713 | KV001: pMVP (L5-L4) eYFP | pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest. |
| 121714 | KP501: pMVP (L5-L4) eYFP-P2A | pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest. |
| 121715 | KO901: pMVP (L5-L4) Blast-P2A | pMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest. |
| 121716 | KP101: pMVP (L5-L4) Puro-P2A | pMVP L5-L4 entry plasmid, contains Puro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Puro selection marker linked by P2A to gene of interest. |
| 121717 | KP001: pMVP (L5-L4) Neo-P2A | pMVP L5-L4 entry plasmid, contains Neo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Neo selection marker linked by P2A to gene of interest. |
| 121718 | KO801: pMVP (L5-L4) Hygro-P2A | pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest. |
| 121719 | KK802: pMVP (L5-L4) Zeo-P2A | pMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest. |
| 121720 | II901: pMVP (L5-L4) FKBP DD | pMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest. |
| 121721 | II801: pMVP (L5-L4) ecDHFR DD | pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest. |
| 121722 | JE301: pMVP (L5-L4) Tir1-P2A-AID | pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest. |
| 121723 | KW701: pMVP (L5-L4) AID | pMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest. |
| 121724 | IO901: pMVP (L5-L4) BioID2 | pMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest. |
| 121725 | IF701: pMVP (L5-L4) APEX2 | pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest. |
| 121726 | IH401: pMVP (L5-L4) CMV promoter | pMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly |
| 121727 | JZ901: pMVP (L5-L4) EF1a promoter | pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly |
| 121728 | HE302: pMVP (L5-L4) RIP | pMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly |
| 121729 | IG101: pMVP (L5-L4) TRE promoter | pMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly |
| 121730 | IO001: pMVP (R4-R3) eGFP | pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly |
| 121731 | IO102: pMVP (R4-R3) eGFP open | pMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121732 | IO201: pMVP (R4-R3) mCherry | pMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly |
| 121733 | HJ601: pMVP (R4-R3) mCherry open | pMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121734 | IO502: pMVP (R4-R3) eCFP | pMVP R4-R3 entry plasmid, contains eCFP for 3- or 4-component MultiSite Gateway Pro assembly |
| 121735 | IO601: pMVP (R4-R3) eCFP open | pMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121736 | IO301: pMVP (R4-R3) eYFP | pMVP R4-R3 entry plasmid, contains eYFP for 3- or 4-component MultiSite Gateway Pro assembly |
| 121737 | IO401: pMVP (R4-R3) eYFP open | pMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121738 | HD301: pMVP (R4-R3) βgal | pMVP R4-R3 entry plasmid, contains βgal for 3- or 4-component MultiSite Gateway Pro assembly |
| 121739 | KH305: pMVP (R4-R3) βgal open | pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121740 | HM001: pMVP (R4-R3) Cre | pMVP R4-R3 entry plasmid, contains Cre for 3- or 4-component MultiSite Gateway Pro assembly |
| 121741 | JN402: pMVP (R4-R3) Firefly Luciferase | pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly |
| 121742 | KJ101: pMVP (R4-R3) osTir1 | pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly |
| 121743 | KJ201: pMVP (R4-R3) osTir1 open | pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121744 | IG510: pMVP (R4-R3) human PDX1 | pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly |
| 121745 | JH204: pMVP (R4-R3) human PDX1 open | pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121746 | HB901: pMVP (L3-L2) polyA | pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly |
| 121747 | HD201: pMVP (L3-L2) IRES-eGFP + polyA | pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence. |
| 121748 | IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA | pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter |
| 121749 | KG901: pMVP (L3-L2) HA epitope tag + polyA | pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121750 | IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA | pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121751 | IX601: pMVP (L3-L2) FLAG epitope tag + polyA | pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121752 | IX503: pMVP (L3-L2) OLLAS epitope tag + polyA | pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121753 | IX301: pMVP (L3-L2) myc epitope tag + polyA | pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121754 | IX401: pMVP (L3-L2) 6x His epitope tag + polyA | pMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121755 | KG802: pMVP (L3-L2) V5 epitope tag + polyA | pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest. |
| 121756 | KH101: pMVP (L3-L2) HA epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121757 | IX907: pMVP (L3-L2) 3x epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121758 | JA001: pMVP (L3-L2) FLAG epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains FLAG epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121759 | IZ901: pMVP (L3-L2) OLLAS epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121760 | IZ702: pMVP (L3-L2) myc epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121761 | IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121762 | KH001: pMVP (L3-L2) V5 epitope tag + WPRE | pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors. |
| 121763 | LC402: pMVP (L3-L2) eGFP + polyA | pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest. |
| 121764 | KY801: pMVP (L3-L2) P2A-eGFP + polyA | pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest. |
| 121765 | LC501: pMVP (L3-L2) mCherry + polyA | pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest. |
| 121766 | KY901: pMVP (L3-L2) P2A-mCherry + polyA | pMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest. |
| 121767 | LC701: pMVP (L3-L2) eCFP + polyA | pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest. |
| 121768 | KZ101: pMVP (L3-L2) P2A-eCFP + polyA | pMVP L3-L2 entry plasmid, contains eCFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest. |
| 121769 | LC601: pMVP (L3-L2) eYFP + polyA | pMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest. |
| 121770 | KZ001: pMVP (L3-L2) P2A-eYFP + polyA | pMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest. |
| 121771 | LC801: pMVP (L3-L2) eGFP + WPRE | pMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors. |
| 121772 | KZ901: pMVP (L3-L2) P2A-eGFP + WPRE | pMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors. |
| 121773 | LC901: pMVP (L3-L2) mCherry + WPRE | pMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors. |
| 121774 | LA001: pMVP (L3-L2) P2A-mCherry + WPRE | pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors. |
| 121775 | LD101: pMVP (L3-L2) eCFP + WPRE | pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors. |
| 121776 | LA201: pMVP (L3-L2) P2A-eCFP + WPRE | pMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest. |
| 121777 | LD001: pMVP (L3-L2) eYFP + WPRE | pMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors. |
| 121778 | LA102: pMVP (L3-L2) P2A-eYFP + WPRE | pMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors. |
| 121779 | KZ301: pMVP (L3-L2) P2A-Blast + polyA | pMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest. |
| 121780 | KZ501: pMVP (L3-L2) P2A-Puro + polyA | pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest. |
| 121781 | KZ401: pMVP (L3-L2) P2A-Neo + polyA | pMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest. |
| 121782 | KZ201: pMVP (L3-L2) P2A-Hygro + polyA | pMVP L3-L2 entry plasmid, contains Hygro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene of interest. |
| 121783 | KZ601: pMVP (L3-L2) P2A-Zeo + polyA | pMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest. |
| 121784 | LA401: pMVP (L3-L2) P2A-Blast + WPRE | pMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectors |
| 121785 | LA601: pMVP (L3-L2) P2A-Puro + WPRE | pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors |
| 121786 | LA501: pMVP (L3-L2) P2A-Neo + WPRE | pMVP L3-L2 entry plasmid, contains Neo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to a gene in lentivirus vectors |
| 121787 | LA301: pMVP (L3-L2) P2A-Hygro + WPRE | pMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectors |
| 121788 | LA701: pMVP (L3-L2) P2A-Zeo + WPRE | pMVP L3-L2 entry plasmid, contains Zeo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to a gene in lentivirus vectors. |
| 121789 | KK501: pMVP (L3-L2) FKBP DD + polyA | pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest. |
| 121790 | KK601: pMVP (L3-L2) ecDHFR DD + polyA | pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest. |
| 121791 | KZ701: pMVP (L3-L2) AID + polyA | pMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene |
| 121792 | KN401: pMVP (L3-L2) FKBP DD + WPRE | pMVP L3-L2 entry plasmid, contains FKBP DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene in lentivirus vectors. |
| 121793 | KN501: pMVP (L3-L2) ecDHFR DD + WPRE | pMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectors |
| 121794 | LA802: pMVP (L3-L2) AID + WPRE | pMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors |
| 121795 | IY001: pMVP (L3-L2) BioID2 + polyA | pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest. |
| 121796 | IY202: pMVP (L3-L2) APEX2 + polyA | pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest. |
| 121797 | JD404: pMVP (L3-L2) BioID2 + WPRE | pMVP L3-L2 entry plasmid, contains BioID2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest in lentivirus vectors. |
| 121798 | JD602: pMVP (L3-L2) APEX2 + WPRE | pMVP L3-L2 entry plasmid, contains APEX2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest in lentivirus vectors. |
| 121799 | IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest. |
| 121800 | KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa |
| 121801 | KA501: pMVP (L3-L2) pA; CMV::TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest. |
| 121802 | KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa |
| 121803 | JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest. |
| 121804 | KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa |
| 121805 | KA601: pMVP (L3-L2) pA; EF1a::TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest. |
| 121806 | KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa |
| 121807 | IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest. |
| 121808 | KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa |
| 121809 | KA401: pMVP (L3-L2) pA; RIP::TETa-pA | pMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest. |
| 121810 | KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA | pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa |
| 121811 | LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold | pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion. |
| 121812 | JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold | pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion. |
| 121813 | KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter | pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion. |
| 121814 | KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter | pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion |
| 121815 | JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP | pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion. |
| 121816 | LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold | pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion |
| 121817 | LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold | pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion |
| 121818 | KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter | pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion |
| 121819 | KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter | pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion |
| 121820 | JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP | pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion. |
| 121821 | IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121822 | IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121823 | IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121824 | IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1 | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121825 | IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121826 | IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L | pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121827 | IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS | pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121828 | KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS | pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121829 | KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR | pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121830 | KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB | pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121831 | KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1 | pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121832 | KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core | pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121833 | KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L | pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121834 | KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS | pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly. |
| 121835 | KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS | pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121836 | KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR | pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121837 | KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB | pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121838 | KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1 | pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121839 | KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core | pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121840 | KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L | pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites |
| 121841 | JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression |
| 121842 | KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression |
| 121843 | IQ013: pMVP/Ad-DEST | pMVP destination vector, empty adenovirus vector backbone |
| 121844 | JC001: pMVP/Ad/RGD-DEST | pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone |
| 121845 | JC101: pMVP/Ad/PK-DEST | pMVP destination vector, empty pK fiber-modified adenovirus vector backbone |
| 121846 | JH401: pMVP/Ad/RGD-PK-DEST | pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone |
| 121847 | JC201: pMVP/Ad/5-35-DEST | pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone |
| 121848 | IZ501: pMVP/Lenti-DEST | pMVP destination vector, empty Lentivirus vector backbone |
| 121849 | IY601: pMVP/Lenti/Blast-DEST | pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette |
| 121850 | IZ401: pMVP/Lenti/Neo-DEST | pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette |
| 121851 | JK104: pMVP-DEST | pMVP destination vector, empty expression plasmid backbone |
| 121852 | JL002: pMVP/eGFP-DEST | pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette |
| 121853 | JL202: pMVP/mCherry-DEST | pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette |
| 121854 | KN206: pMVP/eCFP-DEST | pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette |
| 121855 | JK305: pMVP/Blast-DEST | pMVP destination vector, empty expression vector backbone w/ Blast selection cassette |
| 121856 | JL302: pMVP/Puro-DEST | pMVP destination vector, empty expression vector backbone w/ Puro selection cassette |
| 121857 | JL104: pMVP/Neo-DEST | pMVP destination vector, empty expression vector backbone w/ Neo selection cassette |
| 121858 | KJ801: pMVP/Hygro-DEST | pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette |
| 121859 | KK402: pMVP/Zeo-DEST | pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette |
| 121860 | JN607: pMVP/SB-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone |
| 121861 | JT504: pMVP/SB/eGFP-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette |
| 121862 | JT601: pMVP/SB/mCherry-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette |
| 121863 | KN302: pMVP/SB/eCFP-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette |
| 121864 | JN202: pMVP/SB/Blast-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette |
| 121865 | JN302: pMVP/SB/Puro-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette |
| 121866 | JP705: pMVP/SB/Neo-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette |
| 121867 | KK001: pMVP/SB/Hygro-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette |
| 121868 | KK901: pMVP/SB/Zeo-DEST | pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette |
| 121869 | KX002: pMVP/PB-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone |
| 121870 | LB601: pMVP/PB/eGFP-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette |
| 121871 | LB701: pMVP/PB/mCherry-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette |
| 121872 | LB801: pMVP/PB/eCFP-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette |
| 121873 | LB901: pMVP/PB/Blast-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette |
| 121874 | LC201: pMVP/PB/Puro-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette |
| 121875 | LC101: pMVP/PB/Neo-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette |
| 121876 | LC001: pMVP/PB/Hygro-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette |
| 121877 | LC301: pMVP/PB/Zeo-DEST | pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette |
| 131753 | JI401: Ad5 E1A/Hexon qRT-PCR standard | Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA). |
| 131754 | IX001: pBS/Fiber | Plasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors. |
| 131755 | JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression |
| 131756 | LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression |
| 131757 | LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression |
| 131758 | LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA | pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression |