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Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing.
Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB
Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121686HB401: pMVP (L1-L4) CMV promoterpMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly
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121687IF101: pMVP (L1-L4) EF1a promoterpMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly
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121688HB501: pMVP (L1-L4) RIPpMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly
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121689IG001: pMVP (L1-L4) TBG promoterpMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly
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121690II701: pMVP (L1-L4) cTnT promoterpMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assembly
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121691IG603: pMVP (L1-L4) TRE promoterpMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly
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121692IG301: pMVP (L1-R5) CMV promoterpMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly
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121693IY704: pMVP (L1-R5) EF1a promoterpMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly
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121694IG401: pMVP (L1-R5) RIPpMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly
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121695LF601: pMVP (L1-R5) TBG promoterpMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly
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121696LF801: pMVP (L1-R5) cTnT promoterpMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly
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121697IG703: pMVP (L1-R5) TRE promoterpMVP L1-R5 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly
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121698HE401: pMVP (L1-R5) shRNApMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly
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121699IH001: pMVP (L5-L4) HA epitope tagpMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121700IH201: pMVP (L5-L4) 3x HA epitope tagpMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121701IG901: pMVP (L5-L4) FLAG epitope tagpMVP L5-L4 entry plasmid, contains FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121702IO801: pMVP (L5-L4) 3x FLAG epitope tagpMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121703IH101: pMVP (L5-L4) OLLAS epitope tagpMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121704IX104: pMVP (L5-L4) myc epitope tagpMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121705IX203: pMVP (L5-L4) V5 epitope tagpMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121706KG703: pMVP (L5-L4) 6x His epitope tagpMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
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121707KU701: pMVP (L5-L4) eGFPpMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.
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121708KP201: pMVP (L5-L4) eGFP-P2ApMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.
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121709KU801: pMVP (L5-L4) mCherrypMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.
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121710KP301: pMVP (L5-L4) mCherry-P2ApMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.
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121711KU901: pMVP (L5-L4) eCFPpMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.
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121712KP401: pMVP (L5-L4) eCFP-P2ApMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.
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121713KV001: pMVP (L5-L4) eYFPpMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.
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121714KP501: pMVP (L5-L4) eYFP-P2ApMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.
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121715KO901: pMVP (L5-L4) Blast-P2ApMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest.
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121716KP101: pMVP (L5-L4) Puro-P2ApMVP L5-L4 entry plasmid, contains Puro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Puro selection marker linked by P2A to gene of interest.
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121717KP001: pMVP (L5-L4) Neo-P2ApMVP L5-L4 entry plasmid, contains Neo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Neo selection marker linked by P2A to gene of interest.
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121718KO801: pMVP (L5-L4) Hygro-P2ApMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.
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121719KK802: pMVP (L5-L4) Zeo-P2ApMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest.
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121720II901: pMVP (L5-L4) FKBP DDpMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.
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121721II801: pMVP (L5-L4) ecDHFR DDpMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
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121722JE301: pMVP (L5-L4) Tir1-P2A-AIDpMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
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121723KW701: pMVP (L5-L4) AIDpMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.
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121724IO901: pMVP (L5-L4) BioID2pMVP L5-L4 entry plasmid, contains BioID2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term BioID2 to gene of interest.
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121725IF701: pMVP (L5-L4) APEX2pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.
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121726IH401: pMVP (L5-L4) CMV promoterpMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly
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121727JZ901: pMVP (L5-L4) EF1a promoterpMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly
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121728HE302: pMVP (L5-L4) RIPpMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly
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121729IG101: pMVP (L5-L4) TRE promoterpMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly
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121730IO001: pMVP (R4-R3) eGFPpMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly
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121731IO102: pMVP (R4-R3) eGFP openpMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121732IO201: pMVP (R4-R3) mCherrypMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly
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121733HJ601: pMVP (R4-R3) mCherry openpMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121734IO502: pMVP (R4-R3) eCFPpMVP R4-R3 entry plasmid, contains eCFP for 3- or 4-component MultiSite Gateway Pro assembly
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121735IO601: pMVP (R4-R3) eCFP openpMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121736IO301: pMVP (R4-R3) eYFPpMVP R4-R3 entry plasmid, contains eYFP for 3- or 4-component MultiSite Gateway Pro assembly
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121737IO401: pMVP (R4-R3) eYFP openpMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121738HD301: pMVP (R4-R3) βgalpMVP R4-R3 entry plasmid, contains βgal for 3- or 4-component MultiSite Gateway Pro assembly
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121739KH305: pMVP (R4-R3) βgal openpMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121740HM001: pMVP (R4-R3) CrepMVP R4-R3 entry plasmid, contains Cre for 3- or 4-component MultiSite Gateway Pro assembly
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121741JN402: pMVP (R4-R3) Firefly LuciferasepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly
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121742KJ101: pMVP (R4-R3) osTir1pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly
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121743KJ201: pMVP (R4-R3) osTir1 openpMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121744IG510: pMVP (R4-R3) human PDX1pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly
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121745JH204: pMVP (R4-R3) human PDX1 openpMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly
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121746HB901: pMVP (L3-L2) polyApMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly
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121747HD201: pMVP (L3-L2) IRES-eGFP + polyApMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.
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121748IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyApMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter
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121749KG901: pMVP (L3-L2) HA epitope tag + polyApMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121750IA001: pMVP (L3-L2) 3x HA epitope tag+ polyApMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121751IX601: pMVP (L3-L2) FLAG epitope tag + polyApMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121752IX503: pMVP (L3-L2) OLLAS epitope tag + polyApMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121753IX301: pMVP (L3-L2) myc epitope tag + polyApMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121754IX401: pMVP (L3-L2) 6x His epitope tag + polyApMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121755KG802: pMVP (L3-L2) V5 epitope tag + polyApMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
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121756KH101: pMVP (L3-L2) HA epitope tag + WPREpMVP L3-L2 entry plasmid, contains HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121757IX907: pMVP (L3-L2) 3x epitope tag + WPREpMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121758JA001: pMVP (L3-L2) FLAG epitope tag + WPREpMVP L3-L2 entry plasmid, contains FLAG epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121759IZ901: pMVP (L3-L2) OLLAS epitope tag + WPREpMVP L3-L2 entry plasmid, contains OLLAS epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121760IZ702: pMVP (L3-L2) myc epitope tag + WPREpMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121761IZ801: pMVP (L3-L2) 6x His epitope tag + WPREpMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121762KH001: pMVP (L3-L2) V5 epitope tag + WPREpMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
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121763LC402: pMVP (L3-L2) eGFP + polyApMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.
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121764KY801: pMVP (L3-L2) P2A-eGFP + polyApMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.
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121765LC501: pMVP (L3-L2) mCherry + polyApMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.
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121766KY901: pMVP (L3-L2) P2A-mCherry + polyApMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.
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121767LC701: pMVP (L3-L2) eCFP + polyApMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.
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121768KZ101: pMVP (L3-L2) P2A-eCFP + polyApMVP L3-L2 entry plasmid, contains eCFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.
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121769LC601: pMVP (L3-L2) eYFP + polyApMVP L3-L2 entry plasmid, contains eYFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest.
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121770KZ001: pMVP (L3-L2) P2A-eYFP + polyApMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.
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121771LC801: pMVP (L3-L2) eGFP + WPREpMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.
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121772KZ901: pMVP (L3-L2) P2A-eGFP + WPREpMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.
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121773LC901: pMVP (L3-L2) mCherry + WPREpMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors.
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121774LA001: pMVP (L3-L2) P2A-mCherry + WPREpMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.
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121775LD101: pMVP (L3-L2) eCFP + WPREpMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.
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121776LA201: pMVP (L3-L2) P2A-eCFP + WPREpMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.
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121777LD001: pMVP (L3-L2) eYFP + WPREpMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.
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121778LA102: pMVP (L3-L2) P2A-eYFP + WPREpMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.
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121779KZ301: pMVP (L3-L2) P2A-Blast + polyApMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest.
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121780KZ501: pMVP (L3-L2) P2A-Puro + polyApMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.
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121781KZ401: pMVP (L3-L2) P2A-Neo + polyApMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.
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121782KZ201: pMVP (L3-L2) P2A-Hygro + polyApMVP L3-L2 entry plasmid, contains Hygro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene of interest.
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121783KZ601: pMVP (L3-L2) P2A-Zeo + polyApMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.
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121784LA401: pMVP (L3-L2) P2A-Blast + WPREpMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectors
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121785LA601: pMVP (L3-L2) P2A-Puro + WPREpMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors
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121786LA501: pMVP (L3-L2) P2A-Neo + WPREpMVP L3-L2 entry plasmid, contains Neo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to a gene in lentivirus vectors
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121787LA301: pMVP (L3-L2) P2A-Hygro + WPREpMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectors
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121788LA701: pMVP (L3-L2) P2A-Zeo + WPREpMVP L3-L2 entry plasmid, contains Zeo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to a gene in lentivirus vectors.
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121789KK501: pMVP (L3-L2) FKBP DD + polyApMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.
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121790KK601: pMVP (L3-L2) ecDHFR DD + polyApMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
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121791KZ701: pMVP (L3-L2) AID + polyApMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene
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121792KN401: pMVP (L3-L2) FKBP DD + WPREpMVP L3-L2 entry plasmid, contains FKBP DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene in lentivirus vectors.
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121793KN501: pMVP (L3-L2) ecDHFR DD + WPREpMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectors
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121794LA802: pMVP (L3-L2) AID + WPREpMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors
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121795IY001: pMVP (L3-L2) BioID2 + polyApMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.
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121796IY202: pMVP (L3-L2) APEX2 + polyApMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.
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121797JD404: pMVP (L3-L2) BioID2 + WPREpMVP L3-L2 entry plasmid, contains BioID2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest in lentivirus vectors.
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121798JD602: pMVP (L3-L2) APEX2 + WPREpMVP L3-L2 entry plasmid, contains APEX2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest in lentivirus vectors.
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121799IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.
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121800KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
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121801KA501: pMVP (L3-L2) pA; CMV::TETa-pApMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.
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121802KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa
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121803JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
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121804KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa
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121805KA601: pMVP (L3-L2) pA; EF1a::TETa-pApMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.
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121806KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
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121807IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
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121808KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa
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121809KA401: pMVP (L3-L2) pA; RIP::TETa-pApMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.
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121810KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pApMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa
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121811LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffoldpMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
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121812JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffoldpMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
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121813KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoterpMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
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121814KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoterpMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
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121815JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIPpMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
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121816LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffoldpMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
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121817LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffoldpMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
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121818KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoterpMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
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121819KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoterpMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
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121820JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIPpMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
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121821IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLSpMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
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121822IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPRpMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
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121823IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRABpMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
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121824IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
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121825IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300corepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
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121826IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3LpMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
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121827IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLSpMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
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121828KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLSpMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
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121829KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPRpMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
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121830KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRABpMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
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121831KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
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121832KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300corepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
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121833KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3LpMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
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121834KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLSpMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
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121835KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLSpMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121836KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPRpMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121837KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRABpMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121838KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121839KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300corepMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121840KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3LpMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
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121841JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffoldpMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
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121842KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffoldpMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression
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121843IQ013: pMVP/Ad-DESTpMVP destination vector, empty adenovirus vector backbone
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121844JC001: pMVP/Ad/RGD-DESTpMVP destination vector, empty RGD fiber-modified adenovirus vector backbone
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121845JC101: pMVP/Ad/PK-DESTpMVP destination vector, empty pK fiber-modified adenovirus vector backbone
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121846JH401: pMVP/Ad/RGD-PK-DESTpMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone
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121847JC201: pMVP/Ad/5-35-DESTpMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone
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121848IZ501: pMVP/Lenti-DESTpMVP destination vector, empty Lentivirus vector backbone
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121849IY601: pMVP/Lenti/Blast-DESTpMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette
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121850IZ401: pMVP/Lenti/Neo-DESTpMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette
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121851JK104: pMVP-DESTpMVP destination vector, empty expression plasmid backbone
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121852JL002: pMVP/eGFP-DESTpMVP destination vector, empty expression vector backbone w/ eGFP selection cassette
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121853JL202: pMVP/mCherry-DESTpMVP destination vector, empty expression vector backbone w/ mCherry selection cassette
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121854KN206: pMVP/eCFP-DESTpMVP destination vector, empty expression vector backbone w/ eCFP selection cassette
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121855JK305: pMVP/Blast-DESTpMVP destination vector, empty expression vector backbone w/ Blast selection cassette
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121856JL302: pMVP/Puro-DESTpMVP destination vector, empty expression vector backbone w/ Puro selection cassette
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121857JL104: pMVP/Neo-DESTpMVP destination vector, empty expression vector backbone w/ Neo selection cassette
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121858KJ801: pMVP/Hygro-DESTpMVP destination vector, empty expression vector backbone w/ Hygro selection cassette
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121859KK402: pMVP/Zeo-DESTpMVP destination vector, empty expression vector backbone w/ Zeo selection cassette
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121860JN607: pMVP/SB-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone
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121861JT504: pMVP/SB/eGFP-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette
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121862JT601: pMVP/SB/mCherry-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette
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121863KN302: pMVP/SB/eCFP-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette
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121864JN202: pMVP/SB/Blast-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette
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121865JN302: pMVP/SB/Puro-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette
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121866JP705: pMVP/SB/Neo-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette
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121867KK001: pMVP/SB/Hygro-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette
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121868KK901: pMVP/SB/Zeo-DESTpMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette
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121869KX002: pMVP/PB-DESTpMVP destination vector, empty PiggyBac transposon vector backbone
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121870LB601: pMVP/PB/eGFP-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette
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121871LB701: pMVP/PB/mCherry-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette
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121872LB801: pMVP/PB/eCFP-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ eCFP selection cassette
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121873LB901: pMVP/PB/Blast-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette
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121874LC201: pMVP/PB/Puro-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette
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121875LC101: pMVP/PB/Neo-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette
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121876LC001: pMVP/PB/Hygro-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette
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121877LC301: pMVP/PB/Zeo-DESTpMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette
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