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Comparison of Cas9 activators in multiple species.
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G
Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78898pAct:dCas9-VPRExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.
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78899pAct:dCas9-GCN4Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture
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78900pAct:dCas9-scFvExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells
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78901pAct:dCas9-VP64Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells
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78902pAct:MS2-p65-HSFExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells
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78903pAct:dCas9Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells
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78904pAct:MCP-VP64Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells
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78905pCFD3-MS2tail-SAMVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter
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78906pCFD3-MS2tail-ScaffoldVector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter
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79371pcDNA3_3_TOPO_TA-ms2-VP64Recruits VP64 to a compatible gRNA for transcriptional activation
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79372pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLSRecruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation
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79374pLEX_307_ms2_p65_hsf1Recruits p65_hsf1 to compatible gRNAs for transcriptional activation
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79375pLEX_307_ms2_VP64Recruits VP64 to compatible gRNAs for transcriptional activation
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79376pLEX_307_ms2_VPRRecruits VPR to compatible gRNAs for transcriptional activation
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79377pSB700 Cerulean-Weissman scaffoldCas9 gRNA variant to recruit ms2 proteins
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79378pSB700 Cerulean-Zhang2.0Cas9 gRNA variant to recruit ms2 proteins
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79379pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1Cas9 gRNA variant to recruit ms2 proteins
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79380pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2Cas9 gRNA variant to recruit ms2 proteins
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79382pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4Cas9 gRNA variant to recruit ms2 proteins
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79383pSB700 lenti gRNA Cerulean-zhang2.0 Weiss fullCas9 gRNA variant to recruit ms2 proteins
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