Comparison of Cas9 activators in multiple species.
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G
Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed Article
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G
Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78898 | pAct:dCas9-VPR | Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP. |
| 78899 | pAct:dCas9-GCN4 | Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture |
| 78900 | pAct:dCas9-scFv | Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells |
| 78901 | pAct:dCas9-VP64 | Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells |
| 78902 | pAct:MS2-p65-HSF | Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells |
| 78903 | pAct:dCas9 | Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells |
| 78904 | pAct:MCP-VP64 | Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells |
| 78905 | pCFD3-MS2tail-SAM | Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter |
| 78906 | pCFD3-MS2tail-Scaffold | Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter |
| 79371 | pcDNA3_3_TOPO_TA-ms2-VP64 | Recruits VP64 to a compatible gRNA for transcriptional activation |
| 79372 | pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS | Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation |
| 79373 | pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS | Recruits VPR to a compatible Cas9 protein for transcriptional activation |
| 79374 | pLEX_307_ms2_p65_hsf1 | Recruits p65_hsf1 to compatible gRNAs for transcriptional activation |
| 79375 | pLEX_307_ms2_VP64 | Recruits VP64 to compatible gRNAs for transcriptional activation |
| 79376 | pLEX_307_ms2_VPR | Recruits VPR to compatible gRNAs for transcriptional activation |
| 79377 | pSB700 Cerulean-Weissman scaffold | Cas9 gRNA variant to recruit ms2 proteins |
| 79378 | pSB700 Cerulean-Zhang2.0 | Cas9 gRNA variant to recruit ms2 proteins |
| 79379 | pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1 | Cas9 gRNA variant to recruit ms2 proteins |
| 79380 | pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2 | Cas9 gRNA variant to recruit ms2 proteins |
| 79382 | pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4 | Cas9 gRNA variant to recruit ms2 proteins |
| 79383 | pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full | Cas9 gRNA variant to recruit ms2 proteins |