Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J
Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138456 | pHR_TRE3G-p300-dCas9-P2A-mCherry | 2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry |
| 138457 | Lenti_MCP-LSD1_Hygro | lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker |
| 138458 | Lenti_MCP-VP64_Hygro | lenti vector encoding the MS2-VP64 with a 2A Hygro resistance marker |
| 138459 | Lenti_MCP-p300_Hygro | lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker |
| 138460 | Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1 | lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB |
| 138461 | Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1 | lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64 |
| 138462 | pHR_TRE3G-LSD1-dCas9-P2A-mCherry | 2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry |
| 138463 | Lenti_sgRNA(MS2)_zsGreen1 | lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1 |