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Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J
Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138456pHR_TRE3G-p300-dCas9-P2A-mCherry2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry
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138457Lenti_MCP-LSD1_Hygrolenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker
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138458Lenti_MCP-VP64_Hygrolenti vector encoding the MS2-VP64 with a 2A Hygro resistance marker
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138459Lenti_MCP-p300_Hygrolenti vector encoding the MS2-p300 with a 2A Hygro resistance marker
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138460Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB
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138461Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64
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138462pHR_TRE3G-LSD1-dCas9-P2A-mCherry2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry
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138463Lenti_sgRNA(MS2)_zsGreen1lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1
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