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Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human Cells.

Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 10.1021/acssynbio.2c00156. Epub 2022 Sep 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188489SpCas9Expresses FLAG tagged SpCas9
188490HiFi Cas9Expresses FLAG tagged HiFi Cas9
188491SpRY Cas9Expresses FLAG tagged SpRY Cas9
188492AsCas12aExpresses FLAG tagged AsCas12a
188493LbCas12aExpresses FLAG tagged LbCas12a
188494SaCas9Expresses FLAG tagged SaCas9
188495DpbCas12eExpresses FLAG tagged DpbCas12e
188496PlmCas12eExpresses FLAG tagged PlmCas12e
188497Cas12j3Expresses FLAG tagged Cas12j3
188498Cas12j2Expresses FLAG tagged Cas12j2
188499SpdCas9 KRABExpresses FLAG tagged SpdCas9 KRAB
188500HiFi dCas9 KRABExpresses FLAG tagged HiFi dCas9 KRAB
188501SpRY dCas9 KRABExpresses FLAG tagged SpRY dCas9 KRAB
188502AsdCas12a KRABExpresses FLAG tagged AsdCas12a KRAB
188503LbdCas12a KRABExpresses FLAG tagged LbdCas12a KRAB
188504SadCas9 KRABExpresses FLAG tagged SadCas9 KRAB
188505DpbdCas12e KRABExpresses FLAG tagged DpbdCas12e KRAB
188506PlmdCas12e KRABExpresses FLAG tagged PlmdCas12e KRAB
188507dCas12j3 KRABExpresses FLAG tagged dCas12j3 KRAB
188508dCas12j2 KRABExpresses FLAG tagged dCas12j2 KRAB
188509SpdCas9 VPRExpresses FLAG tagged SpdCas9 VPR
188510HiFi dCas9 VPRExpresses FLAG tagged HiFi dCas9 VPR
188511SpRY dCas9 VPRExpresses FLAG tagged SpRY dCas9 VPR
188512AsdCas12a VPRExpresses FLAG tagged AsdCas12a VPR
188513LbdCas12a VPRExpresses FLAG tagged LbdCas12a VPR
188514SadCas9 VPRExpresses FLAG tagged SadCas9 VPR
188515DpbdCas12e VPRExpresses FLAG tagged DpbdCas12e VPR
188516PlmdCas12e VPRExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)
188517dCas12j3 VPRExpresses FLAG tagged dCas12j3 VPR
188518dCas12j2 VPRExpresses FLAG tagged dCas12j2 VPR
188519miniCMV eGFP hPGK BSDBackbone for CRISPR activation (CRISPRa) isogenic PAM testing
188520EFS eGFP hPGK BSDBackbone for CRISPR interference (CRISPRi) isogenic PAM testing

Antibodies from Article