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Efficient genome editing in zebrafish using a CRISPR-Cas system.
Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
42241Zebrafish-gRNA-0001
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42242Zebrafish-gRNA-0002
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42243Zebrafish-gRNA-0003
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42244Zebrafish-gRNA-0004
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42245Zebrafish-gRNA-0005
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42246Zebrafish-gRNA-0006
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42247Zebrafish-gRNA-0007
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42248Zebrafish-gRNA-0008
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42249Zebrafish-gRNA-0009
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42250DR274guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
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42251MLM3613Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters
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42252MLM3639
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