Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42244||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung lab (Addgene plasmid# 42250)
- Backbone size w/o insert (bp) 2147
Vector typeCRISPR ; zebrafish expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert namegRNA-fh site #2
SpeciesD. rerio (zebrafish)
Insert Size (bp)20
Entrez Genefh (a.k.a. Fh1, im:7152785, ns:zf-e152, zf-e152, zgc:66253, zgc:77498)
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Target site: GGAGCGAGCGGAGCGGTACA
Guide RNA sequence: TAATACGACTCACTATAGGAGCGAGCGGAGCGGTACAGTTTTAGAGCTAGAAATAGCAAGT TAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTAAA
For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Zebrafish-gRNA-0004 was a gift from Keith Joung (Addgene plasmid # 42244 ; http://n2t.net/addgene:42244 ; RRID:Addgene_42244)
For your References section:Efficient genome editing in zebrafish using a CRISPR-Cas system. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. 10.1038/nbt.2501 PubMed 23360964