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Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetON-shYAP
Plasmid#115667PurposeInducible YAP knockdownDepositorInsertYAP shRNA (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)
Viral Prep#26973-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV2)
Viral Prep#50465-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…PromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTagBFP2
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorInsertPark2 (PRKN Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)
Viral Prep#83898-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsmCherryAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.1 (AAV5)
Viral Prep#111067-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B95(DE3) ΔA ΔfabR ΔserB
Bacterial Strain#197655PurposeThis strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.DepositorBacterial ResistanceNoneAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-LARGE1-EF1a-BSD
Plasmid#205151PurposeExpress UbC-driven LARGE1 and EF-1a driven BSDDepositorInsertLARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-S-tdTomato
Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_blasti
Plasmid#194067Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV8)
Viral Prep#105540-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only