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Luciano Marraffini Lab Plasmids


The Luciano Marraffini Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA Nucleic Acids Res. 2013 Jun 12.
A ruler protein in a complex for antiviral defense determines the length of small interfering CRISPR RNAs. Hatoum-Aslan A, Samai P, Maniv I, Jiang W, Marraffini LA J Biol Chem. 2013 Sep 27;288(39):27888-97. doi: 10.1074/jbc.M113.499244. Epub 2013 Aug 9.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
42875pCRISPRCRISPR-BsaIBacterial Expression, CRISPR ; E.coliRNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. A crRNA expression plasmid for targeting a specific sequence. pZE21-MCS1 Add to Cart
42876pCas9tracr/Cas9Bacterial Expression, CRISPR ; E.coliRNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide pACYC184 Add to Cart
44505pCRISPR::rpsLCRISPR::rpsLCRISPR ; E.coliRNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. A crRNA expression plasmid specific to the rpsL allele. pZE21-MCS1 rpsL b3342, ECK3329, JW3304, asuB, strA Add to Cart
46569pdCas9tracrRNA (Other), dcas9 (Other), CRISPR arrayBacterial Expression, CRISPRProgrammable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers. pACYC184 Add to Cart
46570pWJ66tracrRNA (Other), dcas9-w (Other), CRISPR arrayBacterial Expression, CRISPRProgrammable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9) pACYC184 Add to Cart
46571pWJ68tracrRNA (Other), w-dcas9 (Other), CRISPR arrayBacterial Expression, CRISPRProgrammable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9) pACYC184 Add to Cart
70038pPS22 (pET23a-Cas10/Csm/csm2H6N)Cas10/Csm complex (Other)Bacterial ExpressionA ruler protein in a complex for antiviral defense determines the length of small interfering CRISPR RNAs. J Biol Chem. 2013 Sep 27;288(39):27888-97. doi: 10.1074/jbc.M113.499244. Epub 2013 Aug 9. Bacterial expression of the S. epidermidis Cas10·Csm complex pET23a Add to Cart