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pgRNA-humanized Citations (38)

Originally described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
PubMed Journal

Articles Citing pgRNA-humanized

CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS, Qi LS. Nat Protoc. 2013 Nov;8(11):2180-96. doi: 10.1038/nprot.2013.132. Epub 2013 Oct 17. PubMed
Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Gao X, Tsang JC, Gaba F, Wu D, Lu L, Liu P. Nucleic Acids Res. 2014 Nov 10;42(20):e155. doi: 10.1093/nar/gku836. Epub 2014 Sep 15. PubMed
Mature T cell responses are controlled by microRNA-142. Sun Y, Oravecz-Wilson K, Mathewson N, Wang Y, McEachin R, Liu C, Toubai T, Wu J, Rossi C, Braun T, Saunders T, Reddy P. J Clin Invest. 2015 Jul 1;125(7):2825-40. doi: 10.1172/JCI78753. Epub 2015 Jun 22. PubMed
The DNA Structure-Specific Endonuclease MUS81 Mediates DNA Sensor STING-Dependent Host Rejection of Prostate Cancer Cells. Ho SS, Zhang WY, Tan NY, Khatoo M, Suter MA, Tripathi S, Cheung FS, Lim WK, Tan PH, Ngeow J, Gasser S. Immunity. 2016 May 17;44(5):1177-89. doi: 10.1016/j.immuni.2016.04.010. Epub 2016 May 10. PubMed
Benchmarking of TALE- and CRISPR/dCas9-Based Transcriptional Regulators in Mammalian Cells for the Construction of Synthetic Genetic Circuits. Lebar T, Jerala R. ACS Synth Biol. 2016 Oct 21;5(10):1050-1058. Epub 2016 Jun 28. PubMed
Editing DNA Methylation in the Mammalian Genome. Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R. Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056. PubMed

Associated Plasmids

ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Abeta Secretion. Huang YA, Zhou B, Wernig M, Sudhof TC. Cell. 2017 Jan 26;168(3):427-441.e21. doi: 10.1016/j.cell.2016.12.044. Epub 2017 Jan 19. PubMed
Targeting of Polycomb Repressive Complex 2 to RNA by Short Repeats of Consecutive Guanines. Wang X, Goodrich KJ, Gooding AR, Naeem H, Archer S, Paucek RD, Youmans DT, Cech TR, Davidovich C. Mol Cell. 2017 Mar 16;65(6):1056-1067.e5. doi: 10.1016/j.molcel.2017.02.003. PubMed
A G-quadruplex structure at the 5' end of the H19 coding region regulates H19 transcription. Fukuhara M, Ma Y, Nagasawa K, Toyoshima F. Sci Rep. 2017 Apr 3;8:45815. doi: 10.1038/srep45815. PubMed
Minute Virus of Mice Inhibits Transcription of the Cyclin B1 Gene during Infection. Fuller MS, Majumder K, Pintel DJ. J Virol. 2017 Jun 26;91(14). pii: JVI.00428-17. doi: 10.1128/JVI.00428-17. Print 2017 Jul 15. PubMed
Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase. Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, Fukuyama T, Uematsu T, Kobayashi N, Kimura H, Yamagata K. PLoS One. 2017 May 18;12(5):e0177764. doi: 10.1371/journal.pone.0177764. eCollection 2017. PubMed
Reduction of infection by inhibiting mTOR pathway is associated with reversed repression of type I interferon by porcine reproductive and respiratory syndrome virus. Liu Q, Miller LC, Blecha F, Sang Y. J Gen Virol. 2017 Jun;98(6):1316-1328. doi: 10.1099/jgv.0.000802. Epub 2017 Jun 14. PubMed
Integrative analysis of genomic alterations in triple-negative breast cancer in association with homologous recombination deficiency. Kawazu M, Kojima S, Ueno T, Totoki Y, Nakamura H, Kunita A, Qu W, Yoshimura J, Soda M, Yasuda T, Hama N, Saito-Adachi M, Sato K, Kohsaka S, Sai E, Ikemura M, Yamamoto S, Ogawa T, Fukayama M, Tada K, Seto Y, Morishita S, Hazama S, Shibata T, Yamashita Y, Mano H. PLoS Genet. 2017 Jun 21;13(6):e1006853. doi: 10.1371/journal.pgen.1006853. eCollection 2017 Jun. PubMed
Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Nucleic Acids Res. 2017 Jun 29. doi: 10.1093/nar/gkx572. PubMed
Hyper-O-GlcNAcylation induces cisplatin resistance via regulation of p53 and c-Myc in human lung carcinoma. Luanpitpong S, Angsutararux P, Samart P, Chanthra N, Chanvorachote P, Issaragrisil S. Sci Rep. 2017 Sep 6;7(1):10607. doi: 10.1038/s41598-017-10886-x. PubMed
Inhibition of O-GlcNAcase Sensitizes Apoptosis and Reverses Bortezomib Resistance in Mantle Cell Lymphoma through Modification of Truncated Bid. Luanpitpong S, Chanthra N, Janan M, Poohadsuan J, Samart P, U-Pratya Y, Rojanasakul Y, Issaragrisil S. Mol Cancer Ther. 2018 Feb;17(2):484-496. doi: 10.1158/1535-7163.MCT-17-0390. Epub 2017 Nov 22. PubMed
Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R. Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012. PubMed

Associated Plasmids

Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology. Ziller MJ, Ortega JA, Quinlan KA, Santos DP, Gu H, Martin EJ, Galonska C, Pop R, Maidl S, Di Pardo A, Huang M, Meltzer HY, Gnirke A, Heckman CJ, Meissner A, Kiskinis E. Cell Stem Cell. 2018 Apr 5;22(4):559-574.e9. doi: 10.1016/j.stem.2018.02.012. Epub 2018 Mar 15. PubMed
Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9. Lebar T, Jerala R. Methods Mol Biol. 2018;1772:191-203. doi: 10.1007/978-1-4939-7795-6_10. PubMed
CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-kappaB-Mediated Inhibition of TLR2 Expression in Macrophages. Qian Y, Chen C, Ma L, Wang Z, Wang LF, Zuo L, Yang Y, Huang X, Jiang M, Wang X, Shi H, Fu M, Deng KY, Xin HB. Mediators Inflamm. 2018 May 27;2018:8736949. doi: 10.1155/2018/8736949. eCollection 2018. PubMed
Parvovirus minute virus of mice interacts with sites of cellular DNA damage to establish and amplify its lytic infection. Majumder K, Wang J, Boftsi M, Fuller MS, Rede JE, Joshi T, Pintel DJ. Elife. 2018 Jul 20;7. pii: 37750. doi: 10.7554/eLife.37750. PubMed
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A. Cell. 2018 Dec 13;175(7):1958-1971.e15. doi: 10.1016/j.cell.2018.10.024. Epub 2018 Nov 15. PubMed
Long noncoding RNA NEAT1 mediates neuronal histone methylation and age-related memory impairment. Butler AA, Johnston DR, Kaur S, Lubin FD. Sci Signal. 2019 Jul 2;12(588). pii: 12/588/eaaw9277. doi: 10.1126/scisignal.aaw9277. PubMed
The fundamental role of chromatin loop extrusion in physiological V(D)J recombination. Zhang Y, Zhang X, Ba Z, Liang Z, Dring EW, Hu H, Lou J, Kyritsis N, Zurita J, Shamim MS, Presser Aiden A, Lieberman Aiden E, Alt FW. Nature. 2019 Sep;573(7775):600-604. doi: 10.1038/s41586-019-1547-y. Epub 2019 Sep 11. PubMed
CRISPRa-mediated FOXP3 gene upregulation in mammalian cells. Forstneric V, Oven I, Ogorevc J, Lainscek D, Praznik A, Lebar T, Jerala R, Horvat S. Cell Biosci. 2019 Nov 21;9:93. doi: 10.1186/s13578-019-0357-0. eCollection 2019. PubMed
Partial FMRP expression is sufficient to normalize neuronal hyperactivity in Fragile X neurons. Graef JD, Wu H, Ng C, Sun C, Villegas V, Qadir D, Jesseman K, Warren ST, Jaenisch R, Cacace A, Wallace O. Eur J Neurosci. 2020 May;51(10):2143-2157. doi: 10.1111/ejn.14660. Epub 2020 Feb 4. PubMed
A rare variant of African ancestry activates 8q24 lncRNA hub by modulating cancer associated enhancer. Walavalkar K, Saravanan B, Singh AK, Jayani RS, Nair A, Farooq U, Islam Z, Soota D, Mann R, Shivaprasad PV, Freedman ML, Sabarinathan R, Haiman CA, Notani D. Nat Commun. 2020 Jul 17;11(1):3598. doi: 10.1038/s41467-020-17325-y. PubMed
Males and females differ in the regulation and engagement of, but not requirement for, protein degradation in the amygdala during fear memory formation. Devulapalli R, Jones N, Farrell K, Musaus M, Kugler H, McFadden T, Orsi SA, Martin K, Nelsen J, Navabpour S, O'Donnell M, McCoig E, Jarome TJ. Neurobiol Learn Mem. 2021 Apr;180:107404. doi: 10.1016/j.nlm.2021.107404. Epub 2021 Feb 18. PubMed
Silencing of the TRIM58 Gene by Aberrant Promoter Methylation is Associated with a Poor Patient Outcome and Promotes Cell Proliferation and Migration in Clear Cell Renal Cell Carcinoma. Gan Y, Cao C, Li A, Song H, Kuang G, Ma B, Zhang Q, Zhang Q. Front Mol Biosci. 2021 Mar 16;8:655126. doi: 10.3389/fmolb.2021.655126. eCollection 2021. PubMed
FOXO1 controls protein synthesis and transcript abundance of mutant polyglutamine proteins, preventing protein aggregation. Furtado GV, Yang J, Wu D, Papagiannopoulos CI, Terpstra HM, Kuiper EFE, Krauss S, Zhu WG, Kampinga HH, Bergink S. Hum Mol Genet. 2021 May 31;30(11):996-1005. doi: 10.1093/hmg/ddab095. PubMed
Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system. Farooq U, Notani D. STAR Protoc. 2021 Oct 22;2(4):100857. doi: 10.1016/j.xpro.2021.100857. eCollection 2021 Dec 17. PubMed
Epigenetic interaction between UTX and DNMT1 regulates diet-induced myogenic remodeling in brown fat. Li F, Jing J, Movahed M, Cui X, Cao Q, Wu R, Chen Z, Yu L, Pan Y, Shi H, Shi H, Xue B. Nat Commun. 2021 Nov 25;12(1):6838. doi: 10.1038/s41467-021-27141-7. PubMed
Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats. McFadden T, Farrell K, Martin K, Musaus M, Jarome TJ. Nutr Neurosci. 2022 Mar 13:1-13. doi: 10.1080/1028415X.2022.2046965. PubMed
Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer. Lee KM, Lin CC, Servetto A, Bae J, Kandagatla V, Ye D, Kim G, Sudhan DR, Mendiratta S, Gonzalez Ericsson PI, Balko JM, Lee J, Barnes S, Malladi VS, Tabrizi S, Reddy SM, Yum S, Chang CW, Hutchinson KE, Yost SE, Yuan Y, Chen ZJ, Fu YX, Hanker AB, Arteaga CL. Cancer Immunol Res. 2022 Jul 1;10(7):829-843. doi: 10.1158/2326-6066.CIR-21-0826. PubMed
Binding of the transcription activator-like effector augments transcriptional regulation by another transcription factor. Leben K, Strmsek Z, Lebar T, Verbic A, Dragovan M, Omersa N, Anderluh G, Jerala R. Nucleic Acids Res. 2022 Jun 24;50(11):6562-6574. doi: 10.1093/nar/gkac454. PubMed
Long non-coding RNA DARS-AS1 promotes tumor progression by directly suppressing PACT-mediated cellular stress. Yang L, Lin K, Zhu L, Wang H, Teng S, Huang L, Zhou S, Zhang G, Lu ZJ, Wang D. Commun Biol. 2022 Aug 15;5(1):822. doi: 10.1038/s42003-022-03778-y. PubMed
Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenous Mammalian Genes. Lebar T, Jerala R. Methods Mol Biol. 2023;2577:197-209. doi: 10.1007/978-1-0716-2724-2_14. PubMed
The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis. Kuiper EFE, Gallardo P, Bergsma T, Mari M, Kolbe Musskopf M, Kuipers J, Giepmans BNG, Steen A, Kampinga HH, Veenhoff LM, Bergink S. Nat Cell Biol. 2022 Nov;24(11):1584-1594. doi: 10.1038/s41556-022-01010-x. Epub 2022 Oct 27. PubMed

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