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Andrea Ventura Lab Plasmids


The Andrea Ventura Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22.
Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Vidigal JA, Ventura A Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
64071Adeno EAU6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9 (Mus musculus)AdenoviralIn vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4 pacAd5 Add to Cart
64072Adeno Cas9AdenoviralIn vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. Adenoviral vector for the expression of the Cas9 pacAd5
  • Tag / Fusion Protein
    • FLAG
  • Add to Cart
    64073pX333Mammalian ExpressionIn vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter. pX333
  • Tag / Fusion Protein
    • 3XFLAG-Cas9 (N terminal on backbone)
  • Add to Cart
    69312pDonor_hU6hU6 promoter and sgRNA scaffold flanked by BbsI sitesCRISPRRapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. sgRNA scaffold and human U6 promoter pBluescript KS+ Add to Cart
    69350pDonor_mU6mU6 promoter and sgRNA scaffold flanked by BbsI sitesCRISPRRapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. sgRNA scaffold and mouse U6 promoter Topo Blunt II Add to Cart
    69351pDonor_sU6sU6 promoter and sgRNA scaffold flanked by BbsI sitesCRISPRRapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries. Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. sgRNA scaffold and synthetic U6 promoter Topo Blunt II Add to Cart